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中药漏芦提取工艺的优化
引用本文:侯柏玲,孟大利,李宁,李铣. 中药漏芦提取工艺的优化[J]. 沈阳药科大学学报, 2007, 24(6): 371-373
作者姓名:侯柏玲  孟大利  李宁  李铣
作者单位:沈阳药科大学,中药学院,辽宁,沈阳,110016
摘    要:
目的优化中药祁州漏芦的提取工艺。方法采用正交实验法,考察提取溶媒、提取次数和提取时间对提取效率的影响。以β-蜕皮甾酮含量为指标,采用HPLC法测定提取物含量。结果祁州漏芦的最佳提取工艺条件为用质量分数为70%的乙醇提取3次,第1次8倍量乙醇,提取1.5 h,第2次6倍量乙醇,提取1.0 h时,第3次4倍量乙醇,提取1.0 h。按照筛选出的最佳工艺条件进行了3次重复性实验,测得总β-蜕皮甾酮含量质量分数为0.84%,与预测值基本一致。结论所确定的最佳提取工艺可合理提取漏芦中的活性成分。

关 键 词:祁州漏芦  正交实验  提取工艺
文章编号:1006-2858(2007)06-0371-03
修稿时间:2006-09-14

Optimization for extraction of Rhaponticum uniflorum (L.)DC.
HOU Bai-ling,MENG Da-li,LI Ning,LI Xian. Optimization for extraction of Rhaponticum uniflorum (L.)DC.[J]. Journal of Shenyang Pharmaceutical University, 2007, 24(6): 371-373
Authors:HOU Bai-ling  MENG Da-li  LI Ning  LI Xian
Affiliation:School of Traditional Chinese Materia Medica , Shenyang Pharmaceutical University, Shenyang 110016, China
Abstract:
Objective To optimize the conditions for the extraction of Rhaponticum uniflorum(L.)DC.Methods The conditions for the extraction were studied by the orthogonal test,and the weight of extract were detected taking content of ecdysterone as index.Results The optimum condition for the extraction of Rhaponticum uniflorum(L.)DC.was as follow:70% alcohol was chosen as solvent.Firstly,8 times the amount of alcohol was added,the mixture was kept refluxing for 1.5 h;next,6 times the amount of alcohol for 1.0 h;finally,4 times the amount of alcohol for 1.0 h.Conclusions The optimized method can be applied for the extraction of active components in Rhaponticum uniflorum(L.)DC.
Keywords:Rhaponticum uniflorum(L.)DC.  orthogonal test  extraction process
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