骨水泥中的硫酸钡对破骨细胞的作用

目的验证骨水泥中的硫酸钡对破骨细胞形成及其生物学活性的影响。方法体外培养外周血单核细胞并加入巨噬细胞克隆集落刺激因子及核因子B激活因子配体诱导破骨细胞分化,实验组中分别加入含有或不含有硫酸钡的骨水泥颗粒。以抗酒石酸酸性磷酸酶阳性多核细胞及象牙磨片上虫蚀样骨吸收陷窝的形成昨作为检测破骨细胞形成及其骨吸收活性的检测指标,检验骨水泥中的硫酸钡对破骨细胞的影响。结果含或不含硫酸钡的骨水泥颗粒组抗酒石酸酸性磷酸酶阳性多核细胞形成均早于无骨水泥颗粒的对照组（4天vs6天）,而骨水泥颗粒中是否含有硫酸钡的两组间抗酒石酸酸性磷酸酶阳性多核细胞形成的时间无明显差异。各组间抗酒石酸酸性磷酸酶阳性多核细胞的数量无显著差异;含硫酸钡的骨水泥颗粒组象牙磨片上骨吸收陷窝的面积较不含硫酸钡的骨水泥颗粒组及阴性对照组均增大（28.26±4.98vs22.28±3.49vs14.58±2.82,〈0.05）。结论骨水泥颗粒中的硫酸钡能够促进破骨细胞分化并促进成熟破骨细胞的骨吸收活性。

Effect of BaSO4 in bone cement on osteoclast differentiation and bone resorption

Objective To study the effect of BaSO4 in bone cement on osteoclast formation and bone resorption.Methods Mouse peripheral blood monocytes were cultured in the presence of macrophage-colony stimulating factor and Receptor Activator for Nuclear Factor %cB Ligand in vitro.Polymethylmethacrylate盉aSO4 particles were also added into experimental groups respectively.At the end of the culture,cells cultured on coverslips were fixed and stained for tartrate resistant acid phosphatase,which is a marker of osteoclast.The number of tartrate resistant acid phosphatase positive multinucleated cells on coverslips was counted and compared in each group.Lacuna resorption on dentine slices,which is a functional marker of osteoclast,was also analyzed using light microscopy.The percentage of lacuna resorption area on dentine slices in each group was also compared.Results There was no difference in the number of TRAP positive MNCs among the groups,while lacuna resorption area on dentine slices in Polymethylmethacrylate＋BaSO4 group was significantly increased compared to the other two groups（ 28.26±.98 vs 22.28±.49 vs 14.58±.82,P0.05）.The time of appearance of TRAP positive MNCs and lacuna resorption pits was also brought ahead in the particles groups compared to the control,while no markedly difference was observed between PMMA±aSO4groups.Conclusion BaSO4 in bone cement promotes osteoclast differentiation and bone resorption.