以PLGA为支架的组织工程脂肪的实验研究

目的：研究以PLGA为支架材料形成的组织工程脂肪作为软组织填充材料的可行性。方法：实验组：取兔的脂肪间充质干细胞，行体外扩增培养至第3代，达到一定数量后成脂诱导1周，接种于PLGA支架上，继续诱导1周，置于兔背部肩胛骨两侧皮下，分别于4、8周从大体和组织学方面观察脂肪形成情况；对照组：单纯以未接种细胞的支架移植于兔背部肩胛骨两侧皮下，于相同时间点进行检测。结果：实验组植入4周后，该复合物在支架中有脂肪形成，并可见细胞突入支架材料中；8周后支架材料继续降解，脂肪组织形成明显，伴有少量血管长入。对照组支架材料逐渐崩解，未见脂肪组织形成。结论：以PLGA为支架材料形成的组织工程脂肪作为软组织填充材料提供了一个好的思路。

Experimental study on PLGA as scaffold for tissue engineering adipose

Objective:To study on poly(lactide-co-glycolide) acid(PLGA) as scaffold for adipose tissue engineering.Methods: The adipocytes isolated from adipose tissue of rabbits were cultured in vitro and harvested,and then seeded into PLGA scaffold.After incubated for 1 week,the complexes were implanted into the subperiosteum tissue of the back of the rabbits.The specimens were dissected and examined macroscopically and histologically 4 and 8 weeks later.The PLGA scaffold without adipocytes implanted into the subperiosteum tissue of the rabbits was used as control group.Results: New mature adipose was observed in the scaffold in the experimental group,and the histological examination revealed the proliferated adipocytes and extracelluar matrixes.No new mature adipose was observed in the control group.Conclusion: Engineered-adipose using PLGA as the scaffold can be used as the implanted material in adipose tissue engineering.