| 二烯丙基二硫对人白血病HL-60细胞株survivin表达的影响 |
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| 引用本文： | 于明霞,范自力,姚晨姣. 二烯丙基二硫对人白血病HL-60细胞株survivin表达的影响[J]. 现代医药卫生, 2007, 23(21): 3166-3167 |
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| 作者姓名： | 于明霞 范自力 姚晨姣 |
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| 作者单位： | 中南大学湘雅医学院附属三医院血液内科,湖南,长沙,410013 |
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| 摘 要： | 目的:观察二烯丙基二硫(diallyl disulfide,DADS)作用于人白血病HL-60细胞株后,细胞增殖及survivin蛋白表达的变化,探讨DADS对HL-60细胞增殖的影响及其机制。方法:DADS作用HL-60后,MTT法检测细胞增殖情况,SP-免疫组化法检测细胞survivin蛋白表达的变化。结果:DADS可以呈时间-浓度依赖性抑制HL-60细胞增殖并下调survivin蛋白的表达。结论:DADS能通过下调survivin蛋白的表达而抑制HL-60细胞的增殖。
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| 关 键 词： | 二烯丙基二硫 HL-60细胞株 Survivin |
| 文章编号： | 1009-5519（2007）21-3166-02 |
| 修稿时间： | 2007-07-19 |
Effect of diallyl disulfide on the expression of survivin protein in human leukemia HL-60 cell line |
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| YU Ming-xia,FAN Zi-li,YAO Chen-jiao. Effect of diallyl disulfide on the expression of survivin protein in human leukemia HL-60 cell line[J]. JOURNAL OF MODERN MEDICINE & HEALTH, 2007, 23(21): 3166-3167 |
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| Authors: | YU Ming-xia FAN Zi-li YAO Chen-jiao |
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| Affiliation: | Department of Hematology,The Third Affiliated Hospital of Xiangya Medical College ,Central South University , Changsha 410013,China |
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| Abstract: | Objective:To study the proliferation and expression of survivin protein in human leukemia HL-60 cell line treated with diallyl disulfide(DADS) and to thus investigate the mechanism of DADS to inhibit HL-60 cells' growth.Methods:MTT was used to test the growth of HL-60 cells treated by DADS;while SP-immunohistochemistry for the expression of survivin protein in HL-60 cells.Results:DADS could inhibit the proliferation and down-regulate the expression of survivin protein of HL-60 cell line in a dose-time dependent manner.Conclusion:DADS can effectively inhibit the proliferation of HL-60 cell line by down-regulating the level of survivin protein. |
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| Keywords: | Diallyl disulfide HL-60 cell line Survivin |
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