Uptake of Malassezia furfur by human dermal fibroblasts: effect of ketoconazole and cytoskeleton inhibitors |
| |
Authors: | Adone Baroni Brunella Perfetto Iole Paoletti Luisa De Martino Elisabetta Buommino Eleonora Ruocco V. Ruocco |
| |
Affiliation: | (1) Dipartimento di Dermatologia, Facoltà di Medicina e Chirurgia II Università degli Studi di Napoli, Via Pansini, 5, Napoli, Italy Tel.: +39+81-5666828, Fax: +39+81-5468759, IT;(2) Dipartimento di Medicina Sperimentale, Sezione di Microbiologia e Microbiologia Clinica, Facoltà di Medicina e Chirurgia II Università degli Studi di Napoli, Via Costantinopoli 16, Napoli, Italy, IT |
| |
Abstract: | Abstract We showed the ability of human dermal fibroblasts to take up Malassezia furfur and the effect of ketoconazole and cytoskeleton inhibitors, including cytochalasin D and colchicine, on invasivity. Engulfment was evaluated by May Grunwald Giemsa stain and confirmed by acridine orange staining and electron microscopy. Both revealed the different steps of engulfment, including a fusion event between lysosomes and phagosomes containing M. furfur. Subinhibitory concentrations of ketoconazole (5 μg/ml) reduced the invasive capacity compared to controls (52.0 ± 6.3 vs 10.0 ± 1.2). M. furfur induced changes in the cytoskeleton of human dermal fibroblasts, with signs of disaggregation of actin fibres. We also studied the effect of the cytoskeleton inhibitors, cytochalasin D (1 μg/ ml) and colchicine (1 μg/ml), on engulfment. Cytochalasin D, an inhibitor of actin polymers, inhibited the uptake of M. furfur by human dermal fibroblasts. Colchicine, a microtubule inhibitor, reduced the uptake of M. furfur less markedly. This suggests that the process of engulfment is F-actin-dependent, but the integrity of microtubules is also important in “non-professional” phagocytic cells such as dermal fibroblasts. Received: 25 May 2000 / Revised: 26 January 2001 / Accepted: 12 May 2001 |
| |
Keywords: | Malassezia furfur Ketoconazole Cytoskeleton Dermal fibroblast |
本文献已被 SpringerLink 等数据库收录! |
|