腹腔注射攻毒在蜱媒脑炎病毒感染BALB/c小鼠中的应用

目的 研究腹腔注射攻毒在蜱媒脑炎病毒（TBEV）感染BALB/c小鼠中的应用。方法 TBEV以103、104空斑形成单位（PFU）的攻毒剂量经腹腔注射感染BALB/c小鼠，观察小鼠的感染症状、体重变化与生存率。通过H-E染色显示小鼠脑、脾的病理改变，采用免疫组织化学检测小鼠脑、脾中TBEV蛋白的表达，空斑实验检测小鼠脑、脾中病毒滴度的动态变化。结果 TBEV感染第3天小鼠体重开始下降，第6天104 PFU组小鼠出现弓背、后肢瘫痪症状，第7天104 PFU组小鼠开始死亡，第9天103 PFU组小鼠全部死亡。与对照组小鼠相比，感染组小鼠的体重在第5~8天降低具有显著性差异（P<0.05或P<0.001）；小鼠生存率降低差异显著（P<0.01）。TBEV蛋白动态表达于感染组小鼠的脑与脾。103 PFU TBEV感染第7天，小鼠脑内TBEV滴度高达（1.3?0.6）×105 PFU/mL，而脾中滴度为（1.3?0.6）×103 PFU/mL。结论 经腹腔注射攻毒，TBEV在BALB/c小鼠体内建立感染，可增殖并具致病性。

Application of intraperitoneal injection in BALB/c mice infected with tick-borne encephalitis virus

Objective The application of intraperitoneal injection in BALB/c mice infected with tick-borne encephalitis virus (TBEV) was studied. Methods BALB/c mice were infected with TBEV by intraperitoneal injection at two doses including103 and 104 plaque-forming unit (PFU) and that infection symptoms, weight changes and survival rates of the mice were monitored. Pathological changes in brain and spleen of the mice were observed by H-E staining. Expression of TBEV protein in brain and spleen of the mice was detected by immunohistochemistry. Plaque assay was used to determine the dynamic changes in virus titers in brain and spleen of the mice. Results The infected mice began to lose weight on day 3, developed arched back and hind limb paralysis on day 6 in the 104 PFU group, died on day 7, and all died on day 9 post-infection. In comparison with the control group mice, the body weight of mice in the infected group decreased significantly (P<0.05 or P<0.001) on the 5th to 8th day. The survival rate of mice in the infected group also decreased significantly (P<0.01). TBEV protein was dynamically expressed in the brain and spleen of infected mice. On day 7 post-infection with 103 PFU TBEV, virus titers were (1.3?0.6) ×105 PFU/mL in the brain of infected mice and (1.3?0.6) ×103 PFU/mL in the spleen. Conclusion BALB/c mice infected with TBEV was established by intraperitoneal injection challenge and TBEV could proliferate and be pathogenic in the mice.