微 RNA-30d-5p通过调控 ABCB5对胰腺癌进程的影响

目的探讨微 RNA(miR)-30d-5p在胰腺癌( PAAD)中的潜在作用和调控机制。方法选择 2016年 12月至 2019年 1月来新疆维吾尔自治区人民医院接受手术治疗的胰腺癌病人 35例，术后即刻获得胰腺肿瘤组织和癌旁非肿瘤组织，采用实时定量 PCR检测 miR-30d-5p在胰腺癌组织和细胞中的表达水平。使用细胞计数试剂盒( CCK-8)、克隆形成、 transwell和流式细胞术检测 miR-30d-5p对细胞增殖、迁移、侵袭和凋亡的影响。通过萤光素酶测定和蛋白质印迹法评估 miR-30d-5p对 ABCB5表达的调控作用。进一步构建裸鼠成瘤模型通过肿瘤质量和体积验证 miR-30d-5p对肿瘤生长的影响。采用苏木精伊红染色验证肿瘤组织增殖情况。结果与癌旁组织( 1.03±0.17)及正常细胞( 0.98±0.18)相比，胰腺癌组织( 0.35±0.08)和细胞( 0.63±0.08，

MiR-30d-5p affects the proliferation, invasion and metastasis of pancreatic cancer by regulating ABCB5

Objective To explore the potential role and regulatory mechanism of microRNA (miR)-30d-5p in pancreatic adenocarci. noma (PAAD).Methods A total of 35 surgical cases of pancreatic cancer patients were selected from Xinjiang Uygur Autonomous Re.gion People''s Hospital between December 2016 and January 2019 to investigate the immediate gain of pancreatic tumor tissue and ad.jacent carcinoma tissue. Real-time quantitative PCR was employed to detect the expression level of miR-30d-5p in pancreatic cancer tissues and cells. The effects of miR-30d-5p on cell proliferation, migration, invasion, and apoptosis were assessed using cell count. ing kit-8 (CCK-8), colony formation assay, transwell assay, and flow cytometry. Luciferase assay and Western blot analysis were con.ducted to evaluate the regulatory effect of miR-30d-5p on ABCB5 expression. Furthermore, a nude mouse tumor model was established to verify the impact of miR-30d-5p on tumor growth by measuring tumor weight and volume. Hematoxylin and eosin staining was per.formed for histological examination.Results Compared with adjacent tissues (1.03±0.17) and normal cells (0.98±0.18), the expression of miR-30d-5p in pancreatic cancer tissues (0.35±0.08) and cells (0.63±0.08, 0.23±0.07, 0.48±0 .07, and 0.31 ± 005) was significantly decreased (P<0.05); overexpression of miR-30d-5p inhibited the vitality, migration, and invasion of tumor cells while promoting cell apoptosis with statistical significance (P<0.05); inhibition of ABCB5 expression suppressed cell vitality, migration, invasion, and in.duced apoptosis in pancreatic cancer cells with statistical significance (P<0.05); in vivo experiments demonstrated that the miR-30d-5p group could inhibit tumor growth with statistical significance (P<0.05).Conclusion MiR-30d-5p affects pancreatic cancer cell prolif.eration, migration, invasion, and apoptosis by targeting ABCB5. It may serve as a potential prognostic biomarker and target for targetedtherapy in PC patients.