开心解郁汤对抑郁模型大鼠结肠组织的保护作用

目的:观察开心解郁汤对抑郁模型大鼠结肠组织诱导型一氧化氮合酶(iNOS)、谷胱苷肽过氧化物酶(GSH-Px)活性及一氧化氮(NO)含量的影响,探索开心解郁汤保护结肠组织的部分作用机制。方法:将30只SD雄性大鼠随机分为正常组、模型组、开心解郁汤组,每组10只;用慢性应激结合孤养的方式造模21 d。在造模同时,开心解郁汤组给予开心解郁汤ig40 g.kg-1,1次/d,连续21 d。紫外分光光度法检测大鼠结肠组织中iNOS,GSH-Px的活性及NO的含量。结果:与正常组比较,模型组大鼠结肠组织中iNOS活性、NO含量增加,GSH-Px活性明显降低(P<0.05);与模型组比较,开心解郁汤可显著降低抑郁模型大鼠结肠组织中iNOS的活性及NO的含量,提高结肠组织中GSH-Px的活性(P<0.05)。结论:开心解郁汤具有保护抑郁状态下大鼠结肠组织功能的作用,其机制可能与降低结肠组织中iNOS活性及NO含量、提高GSH-Px活性有关。

Effect of Kaixin Jieyu Decoction on Protecting Colon Tissue in Depressed Rats

Objective: To investigate the effects of Kaixin Jieyu Decoction on the activity of inducible nitric oxide synthase(iNOS), glutathione peroxidase(GSH-Px) and the content of nitric oxide(NO) in colon tissue of the depressed rats. So as to reveal partial mechanisms of Kaixin Jieyu Decoction for protecting colon of the depression rats. Method: Thirty male SD rats were evenly randomized into normal,model and Kaixin Jieyu Decoction groups, Depression model of rat was produced by using chronic unpredictable mild stress stimulation (forced ice-water swimming,electric shock,tail-clamping,etc) combined with lonely raising for 21 days.At the same time of producing the depression model rats, Kaixin Jieyu Decoction was given to the Kaixin Jieyu Decoction group rats(40g·kg^-1), once daily,continuously for 21 days.The activity of iNOS, GSH-Px and the content of NO in colon of the depressed rats were detected by ultraviolet spectrophotometer method. Result: The levels of iNOS and NO were increased significantly and the activity of GSH-Px was reduced significantly in colon of the model rats compared to that of the normal group (P<0.05); also the levels of iNOS and NO were reduced significantly, the activity of GSH-Px was increased significantly in colon of Kaixin Jieyu Decoction group compared to that of the model group (P<0.05). Conclusion: Kaixin Jieyu Decoction had protective effect for colon tissue function of the depressed rats.