组织因子途径抑制物对大鼠前DIC状态检测的意义

目的 应用大鼠内毒素血症模型,揭示组织因子途径抑制物(TFPI)在前DIC状态与血小板数(Pt),纤维蛋白原(Fbg),凝血酶原时间(PT),部分凝血活酶时间(APTT)的关系及其意义.方法 采用夹心酶联免疫法测定16只大鼠Pre-DIC状态模型、8只大鼠DIC状态模型、8只健康大鼠血浆TFPI,同时测Pt、Fbg、PT、APTY.结果 大鼠血浆TFPI出现先升高(前DIC状态)后降低(DIC状态),但其数值始终高于正常状态.结论 ,TFPI对内毒素造成的前DIC的监测比常规化验具有灵敏性,对前DIC有早期诊断价值.

The significance of TFPI test in Pre-DIC state of rats

Objective This pre-DIC rat models were constructed by injecting LPS in order to reveal the relationship and significance among tissue factor pathway inhibitor (TFPI),platelet, fibrinogen, prothrombin time and activated partial thromboplastin time. Methods Enzyme-labeled immunosorbent assay was used to detect blood plasma TFPI in pre-DIC state rats(n=16),DIC state rats(n=8)and health rats(n=8).Platelet, fibrinogen, prothrombin time and activated partial thromboplastin time in the same rat were detected. Results TFPI increased first(pre-DIC state)and then decreased(DIC state)in the blood plasma of rata, but the levels of TFPI were always higher than that in the normal state. Conclusions TFPI is more sensitive and accurate to monitor the pro-DIC state than routine examination, which is very useful in the early diagnosis of pre-DIC state.