酪氨酸相关肽的合成及抗孕酮生成活性

利用固相法合成了二十个含羟基氨基酸的小肽。其中,以0.5mol·L^-1二甲二氯硅烷/1.5mol·L^-1苯酚/DCM*为脱除Boc试剂,以TFMSA为切除树脂试剂。经C-18反相柱纯化后,全部产物均通过氨基酸分析要求。体外黄体细胞分泌孕酮实验表明有八个肽化物GlyTyrAlaLys,(SarSer)₂Lys及其申酯,TyrLys,HisTyr-NH₂,ThrProTyrLys-NH₂,TyrThrProArgLys,AspHisProThr-PheLys显示较强的抑制hCG致孕酮分泌的活性,而且前三个肽还能显著抑制基础孕酮的分泌,相反,GlySerTyr能刺激基础孕酮的分泌。目前尚未建立合理的结构一活性关系。

SYNTHESIS OF TYROSINE-RELATED PEPTIDE AND THEIR EFFECT ON PROGESTERONE PRODUCTION

Twenty peptides containing hydroxy- amino acids have been synthesized manually by stepwise solid-phase procedure.The chloromethyl resin and MBHA resin were used as solid supports.A new reagent of 0.5 mol·L^-1 DDSi/1.5mol·L^-1 phenol/ DCM was applied for the removal of Na-Boc group.TFMSA was the cleaving reagent.After purification by C-18 column,allproducts were assayed according to amino acid analysis·The bioactivity of synthetic peptides was tested for the effect on progesterone production in uitro.Eight peptides, GlyTyrAiaLys,( SarSer )₂Lys and its metftyl ester,TyrLys,HisTyr-NH2,ThrProTyrLys-NH₂ TyrThrProArgLys and AspHisProThrPheLys showed significant effect on inhibiting hCG-induced progesterone production,and first three of them could also inhibit basal progesterone secretion. However,peptide GlySerTyr exhibited stimulative activity on basal proges- erone secretion,So far,no reasonable relationship between structure and bioactivity was found。