Oxidation of recombinant methionyl human granulocyte colony stimulating factor |
| |
| Affiliation: | 1. Department of Rehabilitation, Japanese Red Cross Aichi Medical Center Nagoya Daiichi Hospital, Nagoya, Japan;2. Department of Integrated Health Sciences, Nagoya University Graduate School of Medicine, Nagoya, Japan;3. Program in Physical and Occupational Therapy, Nagoya University Graduate School of Medicine, Nagoya, Japan;4. Department of Cardiac Rehabilitation, Gifu Heart Center, Gifu, Japan;5. Yokkaichi Naika Heart Clinic, Yokkaichi, Japan;6. Department of Rehabilitation, Nagoya Tokushukai General Hospital, Kasugai, Japan;7. Department of Cardiology, Fujita Health University Bantane Hospital, Nagoya, Japan;8. Department of Biomolecular Sciences, Field of Omics Health Sciences, Nagoya University Graduate School of Medicine, Nagoya, Japan;9. Department of Cardiology, Aichi Medical University, Nagakute, Japan;1. Faculty of Bioscience Engineering, Department of Food Safety and Food Quality, Research Group Food Chemistry and Human Nutrition – nutriFOODchem (partner in Food2Know), Ghent University, Coupure Links 653, Ghent 9000, Belgium;2. Department of Food and Nutrition, Open University of Tanzania (OUT), P.O. Box 23409, Dar es Salaam, Tanzania;3. Nelson Mandela African Institute of Science and Technology, P.O. Box 447, Arusha, Tanzania;4. Tanzania Food and Drugs Authority, Directorate of Laboratory Services, Mabibo External, P. O. Box 77150, Dar es Salaam, Tanzania;5. Prince Leopold Institute of Tropical Medicine, Department of Public Health, Nutrition and Child Health Unit, Nationalestraat 155, Antwerp 2000, Belgium;1. Flanders research Institute for Agricultural, Fisheries and Food (ILVO), Scheldeweg 68, 9090 Melle, Belgium;1. Department of Hematology/Oncology, Massachusetts General Hospital Cancer Center, Boston, USA;2. Academic Unit of Clinical Oncology, Sheffield Cancer Research Centre, Sheffield, UK;3. Division of Urology, Sunnybrook and Women''s College Health Sciences Centre, Toronto, Canada;4. Department of Medical Oncology, The Queen Elizabeth Hospital, Woodville, Australia;5. Department of Electroradiology, Poznan University of Medical Sciences, Poznan;6. Department of Radiotherapy, Wielkopolskie Centrum Onkologii, Poznan, Poland;7. St John of God Hospital, Subiaco, Australia;8. Department of Medical Oncology, BC Cancer Agency, Vancouver, Canada;9. Department of Hematology/Oncology;10. Global Biostatistical Science, Amgen Inc., Thousand Oaks, USA;11. Department of Medical Oncology, Institut Gustave Roussy, University of Paris Sud, Paris, France |
| |
| Abstract: | ![]()
The oxidation of methionine residues in recombinant methionyl human granulocyte colony stimulating factor with hydrogen peroxide has been investigated. Kinetic data of the oxidation were obtained by using reversed phase-high performance liquid chromatography. The stability-indicating capability of this system was confirmed with micellar electrokinetic capillary chromatography. In the pH range 1.9–7.5, the kobs value for the oxidation process is constant. Above pH 7.5, kobs tends to increase with increasing pH. In the pH range 1.9–11.8, four oxidation products were detected in RP-HPLC. Mass spectrometric analysis revealed that one mono-, one di- and two trioxidation products were formed. Using the cyanogen bromide cleavage method the nature of the oxidation products was determined. The mono-oxidation product is the protein with Met121 oxidized, while the dioxidation product has oxidized Met121 and Met126 residues. The trioxidation products are the proteins with Met121, Met126 and Met137 or Met0, Met121 and Met126 oxidized. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
