ZFHX3基因rs7193343多态性与心房颤动的相关性

目的:研究ZFHX3基因rs7193343多态性与心房颤动(房颤)的相关性。方法:采用前瞻式病例对照研究,按照相关标准入选202例汉族人群,其中房颤患者102例,无房颤患者100例,收集其临床资料和静脉血标本,分别提取基因组DNA,通过聚合酶链反应-限制性片段长度多态性方法和基因测序法检测所有患者的ZFHX3基因rs7193343多态性的基因型。结果:①2组患者ZFHX3基因rs7193343的CC基因型(P=0.023,OR=0.422,95%CI0.198～0.900)、TT基因型(P=0.024,OR=2.475,95%CI1.106～5.541)及等位基因(P=0.017,OR=1.614,95%CI1.089～2.391)的分布均差异有统计学意义。②房颤患者中,TT基因型患者的超敏C反应蛋白水平较CC基因型患者明显高[1.62(0.90～2.90)mg/L∶0.71(0.34～1.09)mg/L,P=0.014],且TT基因型患者的左房前后径和右房横径大小均较CC基因型患者明显增大[(38.2±4.9)mm∶(33.5±4.1)mm,P=0.026;(38.9±6.0)mm∶(33.1±2.6)mm,P=0.003]。结论:在大陆汉族人群中,ZFHX3基因rs7193343多态性与房颤具有显著的相关性,T等位基因和TT基因型是房颤发生发展的危险因子,可通过影响心房肌的结构重构和炎症性改变为房颤的发生、发展提供基质。

Relationship between rs7193343 polymorphism in the zinc finger homeobox 3 gene and atrial fibrillation

Objective:To investigate the potential association of rs7193343 polymorphism in the zinc finger homeobox 3(ZFHX3) gene with atrial fibrillation.Method:Two hundred and two patients were divided into two groups: the atrial fibrillation group(n=102) and the control group(n=100).The rs7193343 polymorphism in the ZFHX3 gene were genotyped by polymerase chain reaction-restriction fragment length polymorphism and gene sequencing approaches.Result:①There were significant differences between the two groups in the distribution of CC(P=0.023,OR=0.422,95%CI 0.198-0.900),TT(P=0.024,OR=2.475,95%CI 1.106-5.541) and allele(P=0.017,OR=1.614,95%CI 1.089-2.391) frequencies.②The level of high sensitivity C-reactive protein in patients with atrial fibrillation carrying TT genotype was higher than that of patients carrying CC genotype [1.62(0.90-2.90)mg/L vs 0.71(0.34-1.09)mg/L,P=0.014].Meanwhile,left atrial dimension and right atrial dimension in patients with atrial fibrillation carrying TT genotype were larger than those of patients carrying CC genotype [(38.2±4.9)mm vs(33.5±4.1)mm,P=0.026;(38.9±6.0)mm vs(33.1±2.6)mm,P=0.003].Conclusion:The rs7193343-T in ZFHX3 gene confers a significant risk of atrial fibrillation in Chinese Han population,by influencing the structure of atrial remodeling and inflammatory changes for the substrate of atrial fibrillation.