首页 | 本学科首页   官方微博 | 高级检索  
     

牙周膜细胞在网格型与无纺型聚乳酸纳米纤维支架材料上体外培养的研究比较
引用本文:张静,梅芳,蔡晴,徐青青,邓旭亮,杨小平. 牙周膜细胞在网格型与无纺型聚乳酸纳米纤维支架材料上体外培养的研究比较[J]. 中国生物医学工程学报, 2009, 28(5)
作者姓名:张静  梅芳  蔡晴  徐青青  邓旭亮  杨小平
作者单位:1. 北京化工大学先进复合材料研究中心,北京,100029
2. 北京大学医学部,北京,100083
3. 北京大学口腔医学院,北京,100044
基金项目:"十一五"国家高技术研究发展(863)计划目标导向课题 
摘    要:
研究对比牙周膜细胞在无纺型和网格型聚乳酸纳米纤维膜上的生长行为,探讨支架结构对细胞生长的影响.采用静电纺丝技术,用金属平板或金属网分别接收,得到无纺型和网格型聚乳酸纳米纤维膜;通过SEM观察两种支架形貌差异,并测试比较它们的力学性能.通过MTT测试和SEM观察,比较无纺型和网格型纳米纤维膜对细胞生长的影响.实验结果:网格型膜的纤维直径平均为500~600 nm;无纺型膜的纤维直径大于网格型膜,平均直径约为700 nm,但网格型膜的拉伸断裂应变略大.牙周细胞与支架联合培养的MTT结果显示,与在聚苯乙烯(TCPS) 培养板上的培养比较,两种纳米纤维膜都显示出促进细胞增殖的效果,其中网格膜的促进效果比无纺膜更加明显.SEM观察的结果显示,细胞无法进入无纺型膜内部生长,而网格型膜中由疏松纤维堆积形成的大孔结构则非常有利于细胞进入支架内部,细胞在后者上生长良好.因此,网格型纳米纤维支架是一种优于纤维为完全无纺排布的支架,更适用于组织工程研究.

关 键 词:静电纺丝  纳米纤维支架  牙周膜细胞  生物相容性

Human Periodontal Ligament Cells Cultured on Lattice-like and Non-woven Electrospun Poly(L-lactide) Nanofiber Scaffolds in vitro
ZHANG Jing,MEI Fang,CAI Qing,XU Qing-Qing,DENG Xu-Liang,YANG Xiao-Ping. Human Periodontal Ligament Cells Cultured on Lattice-like and Non-woven Electrospun Poly(L-lactide) Nanofiber Scaffolds in vitro[J]. Chinese Journal of Biomedical Engineering, 2009, 28(5)
Authors:ZHANG Jing  MEI Fang  CAI Qing  XU Qing-Qing  DENG Xu-Liang  YANG Xiao-Ping
Abstract:
The goal of this study is to investigate the growth behavior of human periodontal ligament cells (hPDLCs) seeded on the poly (L-lactide) (PLLA) nanofibrous matrix. The lattice-like PLLA scaffold was prepared by electrospinning with a metal mesh as collector, while the non-woven PLLA scaffold was collected by a metal plate. The mechanical property of both scaffolds was tested by INSTRO-1121 universal materials test machine followed GB13022-91. The cell compatibility of the electrospun scaffolds was evaluated by MTT-extract assay. The adhesion and proliferation of the cells were examined with scanning electron microscope (SEM). The mean diameter of the fibers in lattice-like PLLA scaffolds was 500~600 nm, larger than the fibers in non-woven PLLA scaffolds whose diameter was around 700 nm, but the two PLLA scaffolds exhibited similar mechanical properties.MTT assay showed that hPDLCs had a higher proliferation rate on lattice-like and non-woven PLLA scaffold in reference to those on TCPS. SEM observation further indicated that the cell could infiltrate into the lattice-like nanofibrous film, while most of the hPDLCs remained on the surface of the non-woven scaffold. Therefore, the lattice-like nanofibrous film showed an advantage over the non-woven nanofibrous scaffolds.
Keywords:electrospinning  nanofiber scaffold  periodontal ligament cells  biocompatibility
本文献已被 万方数据 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号