整合素α2对神经母细胞瘤细胞粘附于胶原蛋白的调节作用

目的：探讨两价阳离子和针对整合素α2的 特异性阻断性单克隆抗体对神经母细胞瘤细胞株SK-N-SH细胞粘附于胶原蛋白（Col）的影响，探讨整合素α2β1对该细胞粘附反应的调节作用。方法:将含有不同M g2+、Ca2+浓度和有或无抗α2单克隆抗体6F1的SK-N-SH细胞混悬液。加入96孔 平底非细胞培养板，此反应板已包被Ⅰ型大鼠尾Col（4 μg/well），所粘附的细胞用BCA方 法测定，以吸光度（A570）代表粘附细胞的数量。结果:Mg 2+能明显增加SK-N-SH细胞Ⅰ型Col的粘附反应；Mg2+浓度为1 mmol/L时，细胞 粘附的反应已接近高峰,与对照组相比A570分别为0.59±0.03和0.2 5±0.0 1 (P＜0.01），而Ca2+对细胞的粘附无明显影响；抗α2单克隆抗体6F1（10 mL /L）能显著阻断SK-N-SH细胞粘附于胶原蛋白(A=0.27±0.01)，其粘附水平接近无Mg 2+的粘附反应水平（A=0.32±0.01）。 结论:整合素α2介导 SK-N-SH细胞对胶原蛋白粘附反应，提示整合素α2可能参与调节神经母细胞瘤的转移。

Effect of integrin α2 on adhesion of neuroblastoma cells to collagen

AIM:To study the effect of integrin α2 on adhesion of SK-N-SH neuroblastoma cells to collagen.METHODS:Adhesion of the SK-N-SH cells to immobilized collagen w as tested with various concentration of Mg2+,Ca2+ and with 10 μg/L anti-α2 monoclonal antibody (mAb) 6F1.A570 was detected as adhesio n cell numbers.RESULTS:Mg2+-dependent adhesion of SK-N-SH cells to type I collagen was increased significantly,with peak adhesion at concentration of 1 mmol/L Mg2+.A570 with or without Mg2+ was 0.59±0.03 and 0.25±0.01 respectively (P<0.01).No effect of Ca2+ was found on SK-N-SH cell adhesion.6F1 (10 mg/L) blocked the adhesion completely.CONCLUSION:Integrin α2 regulates neuroblastoma cells adhesion t o collagen and suggest that it may play a role in tumor cell migration,invasion and metastasis.