乙型肝炎病人及其配偶HBV血清标志物检测

目的比较分析乙型肝炎病人及其配偶HBV血清标志物的检测结果。方法采用ELISA法和荧光PCR法对316例乙型肝炎病人及其配偶进行乙型肝炎五项指标与HBVDNA定量检测。结果HBVDNA的阳性率和含量在HBeAg（＋）与HBeAg（-）组差异均有显著意义（χ^2=31．97、68．79，P〈0．01）；HBsAg的相对滴度与HBVDNA的含量关系不密切。乙型肝炎病人配偶HBV感染率为58．2％，有4例HBVDNA含量〉1．0×10^6 copies／L。结论HBeAg与HBV的复制和HBVDNA的高含量关系密切，而HBsAg的相对滴度则不能反映HBV的复制程度及传染性强弱。配偶间的密切接触可以引起HBV感染，但很少导致HBVDNA在体内的存在和复制。

DETECTION OF SERUM HBV MARKERS IN PATIENTS WITH HEPATITIS B AND THEIR SPOUSES

Objective To compare and analyze the HBV markers detected from patients with hepatitis B and their spouses. Methods Using ELISA and fluorescence PCR, 316 patients and their spouses underwent detection of five items （markers） of hepatitis B and quantitative detection of HBV DNA. Results The differences between HBeAg-positive and HBeAg-negative groups were significant in terms of positive rates and contents of HBV DNA （χ^2 = 31.97,68.79;P〈0.01）. No close association was found between HBsAg titer and contents of HBV DNA. The HBV infection rate of patients＇ spouses was 58. 2 %, HBV DNA contents of four of them being higher than 1.0 × 10^6 copies/L. Conclusion A close relationship exists between HBeAg, HBV replication and high-content HBV DNA, while the relative HBsAg titer can not reflect the extent of HBV replication and the infec- tivity. Close contact between spouses contributes HBV infection, but rarely lead to the existence of HBV DNA in the body and reproduction.