子宫内膜癌组织Syk基因的表达及其甲基化分析

目的探讨脾酪氨酸激酶（Syk）基因在子宫内膜癌组织表达及该基因启动子甲基化与子宫内膜癌发生、发展的关系。方法采用逆转录-聚合酶链反应检测52例子宫内膜癌组织及其癌周正常组织中Syk基因的表达，同时采用巢式双重甲基化特异性PCR（MSP）方法检测该基因启动子甲基化情况。结果Syk基因在子宫内膜癌中的表达显著低于相应的癌周正常组织（P-0．000）；子宫内膜癌有淋巴结转移组Syk基因表达率显著低于无淋巴结转移组（x^2=8．32，P〈0．01）。Syk基因启动子甲基化发生率在子宫内膜癌组织中明显高于相应的癌周正常组织（P=0．000）；子宫内膜癌有淋巴结转移组Syk基因启动子甲基化发生率显著高于无淋巴结转移组（x^2=8．15，P〈0．01）；发生甲基化的肿瘤组织中，均无SykmRNA的表达。结论Syk基因启动子甲基化可能是导致Syk基因失活的原因之一，Syk基因启动子甲基化可能与子宫内膜癌发生发展有关。

ANALYSIS OF THE EXPRESSION OF Syk GENE AND ITS METHYLATION IN ENDOMETRIAL CARCINOMA

Objective To investigate the expression of Syk gene in endometrial carcinoma （EC） and the relationship between hypermethylation of Syk gene promoter region and oncogenesis and development of the malignancy. Methods RT-PCR was used to analyze Syk gene expression in EC tissue and normal paraneoplastic tissue in 52 patients, and Nest duplicated methylation-specific PCR （MSP） to detect methylation of Syk gene promoter region. Results The rate of Syk expression in EC was lower than that in adjacent normal tissue （P=0. 000） ; it was also lower in lymph node metastasis than that without （x^2=8.32,P〈 0.01）. The rate of methylation of Syk gene promoter region in cancer tissue was higher than that in normal adjacent tissue （P= 0. 000）. It was higher in EC with lymph-node metastasis than that without （X^2=8.15 ,P〈0.01）. The expression of Syk mRNA was not detected in methylated tumor tissue. Conclusion Hypermethylation of Syk gene promoter region may be one of the reasons of Syk gene inactivation, which may be related to oncogenesis and development of endometrial carcinoma.