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| 热休克蛋白gp96-肽复合物体外对人肝癌细胞的抗肿瘤效应 | |
| 引用本文： | 吴信华,庄红芹,鞠少卿,戚菁,施维,姚登福.热休克蛋白gp96-肽复合物体外对人肝癌细胞的抗肿瘤效应[J].江苏医药,2011,37(4). |
| 作者姓名： | 吴信华 庄红芹 鞠少卿 戚菁 施维 姚登福 |
| 作者单位： | 1. 南通大学附属医院外科综合实验室,226001 2. 南京大学医药生物技术国家重点实验室 3. 南通大学临床医学研究中心 |
| 基金项目： | 江苏省卫生厅重点项目(H200848); 南通市社会发展科技计划项目(S2008053) |
| 摘 要： | 目的研究热休克蛋白gp96-肽复合物修饰树突状细胞(DC)后对人肝癌细胞的体外特异抗肿瘤效应。方法从人原发性肝癌组织中提取gp96-肽复合物,用其修饰DC细胞后与T淋巴细胞共同培养,以MTT法检测该复合物介导的细胞毒性T细胞(CTL)对不同来源人肝癌细胞的抗肿瘤效应,并与粗提抗原修饰DC细胞组相比较。结果 gp96-肽复合物诱导的CTL对原代肝癌细胞杀伤效率为74.3%,明显高于粗提抗原组(42.5%)和未加抗原组(14.4%)(P<0.01),粗提抗原组对肝癌细胞杀伤效率高于未加抗原组(P<0.01)。且该复合物的抗肿瘤效应具有一定的组织特异性。结论 gp96-肽复合物修饰的DC细胞,在体外能刺激产生特异的抗肿瘤细胞毒性T细胞,因而热休克蛋白gp96在肿瘤免疫治疗中具有重要的实用价值。 |
| 关 键 词： | gp96-肽复合物 树突状细胞 肝癌 |
The anti- hepatoma effects of HSP gp96-peptide complexes in vitro |
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| WU Xinhua,ZHUANG Hongqin,JU Shaoqing,et al..The anti- hepatoma effects of HSP gp96-peptide complexes in vitro[J].Jiangsu Medical Journal,2011,37(4). | |
| Authors: | WU Xinhua ZHUANG Hongqin JU Shaoqing |
| Institution: | WU Xinhua,ZHUANG Hongqin,JU Shaoqing,et al.Surgical Comprehensive Lab oratory,Affiliated Hospital,Nantong University,Nantong 226001,CHINA |
| Abstract: | Objective To investigate the specific anti-hepatoma effects of dendritic cells(DCs) modified by HSP gp96-pepetide complexes in vitro.Methods Gp96-peptide complexes were obtained from hepatocellular carcinoma (HCC) tissues.DCs were modified by the complexes and co-cultured with T lymphocytes.The effects of anti-hepatoma by the complexes mediated CTL were detected by MTT method,and then compared with the effects induced by DCs modified crudely extracted antigen.Results The killing effectiveness rate of primar... |
| Keywords: | gp96-Peptide complexes Dendritic cells Hepatocellular carcinoma |
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