| 红细胞膜蛋白结构变化对膜脂流动性的影响 |
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| 引用本文： | 施岩,杨建中,周子亮. 红细胞膜蛋白结构变化对膜脂流动性的影响[J]. 承德医学院学报, 2002, 19(2): 94-95 |
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| 作者姓名： | 施岩 杨建中 周子亮 |
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| 作者单位： | 承德医学院基础部,河北,承德,067000;承德医学院基础部,河北,承德,067000;承德医学院基础部,河北,承德,067000 |
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| 摘 要： | 目的：研究红细胞膜蛋白的变化对膜脂流动性的影响。方法：从三个不同方面改变膜蛋白结构,即（1）用戊二醛交联膜蛋白分子;（2）用伴刀豆蛋白（ConA）作为配体与红细胞膜上的受体蛋白相作用;（3）用胰蛋白酶降解膜表面蛋白,然后利用荧光分光光度计测量经上述三种方式处理后的红细胞膜的荧光偏振度（P）。结果：经三种方法处理地的红细胞膜的荧光偏振度P值均有所上升,表明膜脂流动性下降。结论：膜蛋白分子的改变将会影响膜脂的流动性。
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| 关 键 词： | 红细胞 膜脂 流动性 荧光偏振 |
| 文章编号： | 1004-6879(2002)02-0094-02 |
| 修稿时间： | 2002-04-05 |
THE EFFECT OF ALTERNATION OF RBC MEMBRANE PROTEIN IN THEIR STRUCTURE ON THE FLUIDITY OF THE LIPID |
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| SHI Yan,YANG Jian zhong,ZHOU Zi liang. THE EFFECT OF ALTERNATION OF RBC MEMBRANE PROTEIN IN THEIR STRUCTURE ON THE FLUIDITY OF THE LIPID[J]. Journal of Chengde Medical College, 2002, 19(2): 94-95 |
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| Authors: | SHI Yan YANG Jian zhong ZHOU Zi liang |
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| Abstract: | Objective:To investigate the effect of change of RBC membrane proteins in structure on the fluidity of the membrane lipid.Methods:By using three kinds of methods to change RBC membrane proteins.which are follows:(1)making the RBC membrane proteins linked with glutaraldehyde.(2)causing the interactions between the RBC membrane receptors and ligands with concanavalin A(ConA).(3)degrading the RBC membrane proteins with trypsin.Then we measured the lipid fluidity of the RBC membrane treated with the above ways by fluorescence polarization technique.Results:The lipid fluidity declined distinctly for the RBC membrane which were treated by the three kinds of methods above.Conclusion:The decrease of membrane lipid fluidity may results from the conformation changes of RBC membrane proteins. |
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| Keywords: | RBC:Membrane lipid Fluidity Fluorescence polarization |
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