| 尘螨的抗原和糖蛋白成分的印渍法鉴定 |
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| 引用本文： | 陈文魁.尘螨的抗原和糖蛋白成分的印渍法鉴定[J].皖南医学院学报,1989,8(3):160-162. |
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| 作者姓名： | 陈文魁 |
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| 作者单位： | 皖南医学院寄生虫学教研室 |
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| 摘 要： | 本文用蛋白质转移电泳法分析粉尘螨(dermatophagoides farinae)浸液中的组分。用转移电泳结合酶免疫分析测得21条具抗原活性电泳条带;结合刀豆蛋白A(ConA)搭桥法测得12条能与ConA结合的糖蛋白电泳条带;结合考马氏亮兰染色法只显出7条蛋白电泳条带。这些结果可能有助于进一步分析和纯化粉尘螨浸液中的抗原。
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| 关 键 词： | 粉尘螨 聚丙烯酰胺凝胶电泳 酶免疫分析 刀豆蛋白A 印渍法 |
IDENTIFICATION OF DUST MITE ANTIGENS AND GLYCOPROTEINS WITH BLOTTING |
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| Chen Wenkui.IDENTIFICATION OF DUST MITE ANTIGENS AND GLYCOPROTEINS WITH BLOTTING[J].Acta Academiae Medicinae Wannan,1989,8(3):160-162. |
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| Authors: | Chen Wenkui |
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| Abstract: | The mite extract of Dcrmatophagoides farinae (Df) was analysed by proteiu blotting. It was shown that 21 electrobands with antigenic activities were identified by enzyme immunoassay (EIA), 12 ConA-binding glycoprtein electrobands by ConA bridge method, and only 7 protein bands by Coomassie brolliant blue G250 staining. These results may be of help to further analysing and purifying the antigens in the dust mite extract |
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| Keywords: | Dermatophagoides farinae etectrophoresis polyacrylamide gel enzyme immunoassay ConA blotting |
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