The effects of coxsackievirus B3 infection on induction of ICAM-1 in cardiac myocytes and on Iymphocyte-myocyte adhesion

objective:To observe the effects of coxsackievirus B3(CoxB3) infection on-induction of intercellu lar adhesion molecule-1 (ICAM-1) in cardiac myocytes and on lymphocyte-myocyte adhesion. Metkods: ICAM 1 expression on cultured neonatal rat cardiac myocytes infected by CoxB3 was determined through enzyme linked immunosorbent assay (ELISA) analysis. Lymphocyte-myocyte adhesion was observed with adherence assay and monoclonal antibodies to adhesion molecule inhibition. Results:ICAM-1 expression on cardiac my ocytes was markedly induced after exposure to CoxB3 100 or 200 TCID50/ml infectious dose for 24~48 h (P<0. 05). Adhesion of rat activated lymphocytes to myocytes was stimulated by CoxB3 infection,and the effects of CoxB3 infection on adherence was significantly inhibited by anti-lymphocyte function antigen-1 (LFA-12)monoclonal antibodies (inhibition rate 40. 6% and 26. 45 % respectively). Couclusion:CoxB3 infec tion promotes lymphocyte-myocyte adhesion through inducing ICAAM-1 expression on cardiac myocytes.