HPLC法同时测定人血清中氯硝西泮、硝西泮、三唑仑浓度

目的建立单步萃取-HPLC法同时测定人血清中硝西泮、氯硝西泮、三唑仑浓度。方法血清样品采用二氯甲烷单步萃取,采用Ultimate XB-C18色谱柱（250.0mm×4.6mm,5μm）,流动相为甲醇∶水∶乙腈=10∶56∶34,流速为1mL/min,检测波长为254nm,柱温为30℃,卡马西平为内标物。结果硝西泮、氯硝西泮、三唑仑的线性范围分别为32.5～650.0μg/L（r=0.9997）、31.5～630.0μg/L（r=0.9999）、74～1480μg/L（r=0.9997）,最低检测限分别为8.13μg/L、7.88μg/L、18.5mg/L,日内精密度和日间精密度良好,回收率较高。结论本法操作简便,结果准确可靠。可满足临床快速监测的需要。

Simultaneous Determination of Natrazepam, Clonazepam, Triazolam in Human Serum Concentration by HPLC

Objective To establish a single step method for the determination of the concentration of the natrazepam（NZ） ,clonazepam（CZ） ,triazolam（TZ） .Methods The serum sample was extracted once with Dichloromethane.The residue was dissolved and analyzed with Ultimate XB-C18（250.0 mm×4.6 mm,5 μm） ,mobilephase was methanol∶water∶ acetonitrile=10∶ 56∶ 34,low rate was 1.0 mL/min.The detective UV wavelength was 254 nm,the column temperature was 30℃,carbamazepine was used as internal standard.Results The linear relation was in the range of 32.5～650.0 μg/L（r=0.999 7） ,31.5～630.0 μg/L（r=0.999 9） and 74～1 480 μg/L（r=0.999 7） .The lowest detectable concentration in plasma was 8.13 μg/L,7.88 μg/L,18.5 mg/L,the intra-day precision and inter-day precision were all good,the average recovery rate was comparatively high.Conclusion The method is rapid,accurate and practical.It can be used as the fast method by TDM.