| HPLC法测定冬凌草甲素的平衡溶解度和表观油水分配系数 |
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| 引用本文： | 徐文,孙进,张婷婷,马博,陈大为,何仲贵. HPLC法测定冬凌草甲素的平衡溶解度和表观油水分配系数[J]. 沈阳药科大学学报, 2007, 24(4): 220-222 |
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| 作者姓名： | 徐文 孙进 张婷婷 马博 陈大为 何仲贵 |
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| 作者单位： | 沈阳药科大学,药学院,辽宁,沈阳,110016 |
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| 基金项目： | 辽宁省高等学校优秀人才支持计划 |
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| 摘 要： | 目的测定冬凌草甲素在各种溶剂中的平衡溶解度以及在正辛醇-水和正辛醇-缓冲液体系中的表观油水分配系数。方法采用HPLC法测定冬凌草甲素的浓度,采用摇瓶法测定冬凌草甲素的表观油水分配系数。结果25℃冬凌草甲素在水中的平衡溶解度为0.75 g.L-1,在碱性磷酸盐缓冲液中的平衡溶解度更低;在常用的油中的溶解度小于1 g.L-1;冬凌草甲素的表观油水分配系数Papp为45.8(lgPapp=1.66)。结论冬凌草甲素的水溶性和脂溶性都较差,并且具有中等的疏水性。
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| 关 键 词： | 冬凌草甲素 平衡溶解度 表观油水分配系数 高效液相色谱法 |
| 文章编号： | 1006-2858(2007)04-0220-03 |
| 修稿时间： | 2006-05-08 |
Determination of equilibrium solubility of oridonin and its apparent oil/water partition coefficient by HPLC |
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| XU Wen,SUN Jin,ZHANG Ting-ting,MA Bo,CHEN Da-wei,HE Zhong-gui. Determination of equilibrium solubility of oridonin and its apparent oil/water partition coefficient by HPLC[J]. Journal of Shenyang Pharmaceutical University, 2007, 24(4): 220-222 |
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| Authors: | XU Wen SUN Jin ZHANG Ting-ting MA Bo CHEN Da-wei HE Zhong-gui |
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| Affiliation: | School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China |
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| Abstract: | Objective To determine the equilibrium solubility of oridonin in various media and its partition coefficients for the n-octanol-water/buffer solution systems.Methods A high performance liquid chromatography was established and used to detect the concentration of oridonin;the partition coefficients for the n-octanol-water/buffer solution systems of oridonin were determined by shaking flask method.Results The equilibrium solubility of oridonin in water was 0.75 g·L-1 at 25 ℃ and had lower values in basic buffer solutions;lgPapp of oridonin was 1.66.Conclusions Oridonin is not soluble in either aqueous media or organic phase,its hydrophobicity is moderate. |
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| Keywords: | oridonin equilibrium solubility apparent oil/water partition coefficient HPLC |
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