艾灸预处理对阿尔茨海默病模型大鼠海马CA1区NGF和BDNF表达的影响

目的：研究艾灸预处理百会和肾俞两穴对阿尔茨海默病（AD）模型大鼠脑组织海马CA1区中神经生长因子（NGF）和脑源性神经营养因子（BDNF）蛋白表达的影响及作用机制。方法：SD雄性成年大鼠40只,随机分为正常组、假手术组、模型组和预艾灸组各10只。预艾灸组采用艾灸百会及肾俞穴40次后,于模型组同时采用双侧海马一次性注射凝聚态Aβ1-42制备AD大鼠模型。以Morris水迷宫试验评价4组大鼠学习记忆能力;免疫组化法观察海马CA1区NGF和BDNF的阳性细胞计数。结果：与正常组和假手术组比较,预艾灸组和模型组学习记忆能力及海马CA1区NGF、BDNF阳性细胞数均明显降低（P〈0.05,0.01）;与模型组比较,预艾灸组则表现为明显升高（P〈0.01）。结论：预艾灸处理百会、肾俞两穴能显著改善AD模型大鼠的学习记忆能力,其机制可能与AD大鼠海马CA1区NGF、BDNF的阳性表达增加有关。

Effect of Moxibustion Pretreatment on Expression of NGF and BDNF in Hippocampus CA1 of Alzheimer Disease Model Rats

Objective： To evaluate the effects of moxibustion pretreatment at Baihui（GV20） and Shenshu（BL23） on the expression of NGF and BDNF in hippocampus CA1 of the Alzheimer disease（AD） model rats.Methods： Forty male adult SD rats were randomly and equally allocated into normal,sham operation,model and moxibustion pretreatment groups.After 40 times of moxibustion pretreatment at Baihui and Shenshu,bilateral hippocampi of moxibustion pretreatment and model rats were one-off injected with condensed Aβ1-42 to establish the rat AD models.Morris＇ water maze test was used to evaluate the learning and memory ability of rats.The expression of NGF and BDNF in the CA1 region of the hippocampus was detected by using immunohistochemical techniques.Results： As compared with normal and sham operation groups,learning and memory ability and the number of NGF and BDNF positive cells in hippocamal CA1 region of AD rats were obviously reduced in the Moxibustion pretreatment and model groups,and those in the Moxibustion pretreatment group were significantly increased as compared with the model group.Conclusion： Moxibustion pretreatment at the Baihui and Shenshu points can improve learning and memory ability of AD rats,which might be related with increased NGF and BDNF expression in the hippocampal CA1 region.