番荔枝子去蛋白多糖制备及其体外降糖作用研究

目的:研究番荔枝子去蛋白多糖对HepG2细胞糖消耗及胰岛素抵抗HepG2细胞糖消耗的影响。方法:水提醇沉法制备番荔枝子粗多糖,经过Sevag法除蛋白得番荔枝子去蛋白多糖,用苯酚-硫酸法检测其总糖含量。取对数生长期的HepG2细胞,分别给予不同浓度的番荔枝子去蛋白多糖,检测其对HepG2细胞葡萄糖消耗的影响;建立高胰岛素抵抗模型,同法测定其对细胞液中葡萄糖消耗的影响。结果:番荔枝子去蛋白多糖能轻度促进HepG2细胞的葡萄糖消耗,其作用与剂量呈正相关;番荔枝子去蛋白多糖能明显促进胰岛素抵抗HepG2细胞葡萄糖消耗作用,在浓度为0.08 mg·mL-1时,效果最佳(P<0.01)。同时,番荔枝子去蛋白多糖与生理胰岛素具有一定的协同作用。结论:番荔枝子去蛋白多糖具有较好的体外降糖作用。

Preparation of Protein-free Polysaccharide from Annonae Squamosae Semen and Its In Vitro Hypoglycemic Effect

The study was aimed to research the influence of glucose consumption of HepG2 cell and insulinresistance of HepG2 cell administrated with protein -free polysaccharide from Annonae Squamosae Semen (ASS). Crude polysaccharide from ASS was prepared by water extraction and alcohol precipitation method. Its protein was removed by sevag method. The content of its total sugar was measured by phenol -sulfuric acid method. Besides, the influences of glucose consumption of HepG2 cell and insulin -resistance of HepG2 cell administrated with different concentrations of protein -free polysaccharide were determined. The result showed that protein-free polysaccharide from ASS can slightly improve the glucose consumption of HepG2 cell, which was related to its concentration. The protein-free polysaccharide from ASS can obviously promote insulin-resistance of HepG2 cell. When the drug concentration was 0.08 mg·mL-1, the effect is the best (P < 0.01). Besides, the protein-free polysaccharide from ASS had certain synergistic effect as physiological insulin. It was concluded that the protein-free polysaccharide from ASS had good in vitro hypoglycemic effect.