人参皂苷Rg1的抗抑郁作用及对突触超微结构的影响

目的观察人参皂苷Rg1的抗抑郁作用及慢性应激对额前皮质区突触超微结构的改变。方法采用慢性温和不可预见性应激(chronic unpredictable mild stress,CUMS)的方法建立大鼠抑郁模型。造模同时给予人参皂苷Rg1(5、10和20 mg·kg-1)和度洛西汀(10 mg·kg-1),每天1次,连续28 d。用强迫游泳和糖水消耗的行为学方法检测大鼠的抑郁行为学变化,电子显微镜观察应激后大鼠额前皮质区突触超微结构的变化。结果与对照组相比,慢性应激大鼠在强迫游泳实验中的不动时间明显增加(P<0.01),糖水偏好实验中的糖水消耗百分比明显降低(P<0.01);电镜观察结果显示模型组大鼠额前皮质区突触超微结构异常,突触前膜的突触囊泡密度减小,突触后膜致密物厚度也减少。与模型组相比,人参皂苷Rg1能明显改善慢性应激大鼠的抑郁行为,改善大鼠额前皮质区突触超微结构。结论人参皂苷Rg1对CUMS诱导的大鼠抑郁行为和额前皮质区突触超微结构的异常有明显的改善作用。

Effects of Ginsenoside Rg1 on anti-depression and synaptic ultrastructure

Aim To observe the anti-depressive effect of Ginsenoside Rg1(Rg1) and the alteration of synaptic ultrastructure in prefrontal cortex(PFC) induced by chronic unpredictable mild stress(CUMS).Methods The rat model of depression was established by CUMS for 28 days.Rg1(5,10,20 mg·kg-1·d-1) and duloxetine(10 mg·kg-1·d-1) were administered under stress procedure.Depressive behaviors were estimated in the forced swim test and sucrose preference test.Synaptic ultrastructure in PFC was examined using electron microscope.Results Compared with control group,stressed rat produced a significant increase of immobility time and a decrease of sucrose intake percentage.The study on synaptic ultrastructure in PFC of model rat showed that density of synaptic vesicle in presynaptic membrane and postsynaptic density were decreased.Treatment of Ginsenoside Rg1 could improve depressive behavior and synaptic ultrastructure of CUMS rat.Conclusions Ginsenoside Rg1 has a robust antidepressive effect and an ameliorating effect on synaptic ultrastructure in PFC in rat CUMS model.