Apoptosis of human tongue squamous cell carcinoma cell (CAL-27) induced
by Lactobacillus sp. A-2 metabolites |
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Authors: | Guoliang ZHANG Jie ZHANG Xinyu WANG Wenqin YANG Zhihui SUN Chaurasia Nitesh KUMAR Hong GUAN Jian GUAN |
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Affiliation: | 1. The Oral and Maxillofacial Surgery of the Stomatological College, Jiamusi University, Jiamusi, Heilongjiang, China.;2. Pharmacy School, Qiqihaer Medical University, Qiqihaer, Heilongjiang, China.;3. Research Center of Microecological Engineering Technology, Qiqihaer Medical University, Qiqihaer, Heilongjiang, China. |
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Abstract: |
ObjectiveTo study the effect of Lactobacillus sp. A-2 metabolites onviability of CAL-27 cells and apoptosis in CAL-27 cells.MethodsLactobacillus sp. A-2 metabolites 1 and 2 (LM1 and LM2) wereobtained by culturing Lactobacillus sp. A-2 in reconstituted whey medium andwhey-inulin medium; the cultured CAL-27 cells were treated with differentconcentrations of LM1 and LM2 (0, 3, 6, 12, 24, 48 mg/mL) and assayed by methylthiazolyltetrazolium (MTT) method; morphological changes of apoptotic cell wereobserved under fluorescence microscopy by acridine orange (Ao) fluorescentstaining; flow cytometry method (FCM) and agarose gel electrophoresis were used todetect the apoptosis of CAL-27 cells treated LM1 and LM2.ResultsThe different concentrations of LM1 and LM2 could restrain the growth of CAL-27cells, and in a dose-dependent manner; the apoptosis of CAL-27 cells was obviouslyinduced and was time-dependent. ConclusionsViability of CAL-27 cells was inhibited by Lactobacillus sp. A-2metabolites; Lactobacillus sp. A-2 metabolites could induceCAL-27 cells apoptosis; study on the bioactive compounds in theLactobacillus sp. A-2 metabolites and their molecularmechanism is in progress. |
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Keywords: | Lactobacillus Metabolism Milk protein Oral cancer Apoptosis |
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