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Apoptosis of human tongue squamous cell carcinoma cell (CAL-27) induced by Lactobacillus sp. A-2 metabolites
Authors:Guoliang ZHANG  Jie ZHANG  Xinyu WANG  Wenqin YANG  Zhihui SUN  Chaurasia Nitesh KUMAR  Hong GUAN  Jian GUAN
Affiliation:1. The Oral and Maxillofacial Surgery of the Stomatological College, Jiamusi University, Jiamusi, Heilongjiang, China.;2. Pharmacy School, Qiqihaer Medical University, Qiqihaer, Heilongjiang, China.;3. Research Center of Microecological Engineering Technology, Qiqihaer Medical University, Qiqihaer, Heilongjiang, China.
Abstract:

Objective

To study the effect of Lactobacillus sp. A-2 metabolites onviability of CAL-27 cells and apoptosis in CAL-27 cells.

Methods

Lactobacillus sp. A-2 metabolites 1 and 2 (LM1 and LM2) wereobtained by culturing Lactobacillus sp. A-2 in reconstituted whey medium andwhey-inulin medium; the cultured CAL-27 cells were treated with differentconcentrations of LM1 and LM2 (0, 3, 6, 12, 24, 48 mg/mL) and assayed by methylthiazolyltetrazolium (MTT) method; morphological changes of apoptotic cell wereobserved under fluorescence microscopy by acridine orange (Ao) fluorescentstaining; flow cytometry method (FCM) and agarose gel electrophoresis were used todetect the apoptosis of CAL-27 cells treated LM1 and LM2.

Results

The different concentrations of LM1 and LM2 could restrain the growth of CAL-27cells, and in a dose-dependent manner; the apoptosis of CAL-27 cells was obviouslyinduced and was time-dependent.

Conclusions

Viability of CAL-27 cells was inhibited by Lactobacillus sp. A-2metabolites; Lactobacillus sp. A-2 metabolites could induceCAL-27 cells apoptosis; study on the bioactive compounds in theLactobacillus sp. A-2 metabolites and their molecularmechanism is in progress.
Keywords:Lactobacillus   Metabolism   Milk protein   Oral cancer   Apoptosis
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