| HSV—1 SM44株糖蛋白D基因真核表达载体的构建及其免？… |
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| 引用本文： | 余颖,马文煜.HSV—1 SM44株糖蛋白D基因真核表达载体的构建及其免？…[J].细胞与分子免疫学杂志,2000,16(4):308-310. |
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| 作者姓名： | 余颖 马文煜 |
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| 作者单位： | 第四军医大学基础部微生物学教研室,陕西西安 |
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| 基金项目： | 国家自然科学基金资助 !No.39770040 |
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| 摘 要： | 目的 构建ＨＳＶ－１型ＳＭ４４株糖蛋白Ｄ（ｇＤ）基因的真核表达载体，并用此重组质粒直接免疫小鼠，探讨ＨＳＶ－１ ｇＤ基因作为基因疫苗的可能性。方法 从ＨＳＶ－１基因组中扩增ｇＤ的全编码基因，克隆入载体ｐＵＣ１９中，测序鉴定后转入真核表达载体ｐｃＤＮＡ３．１（＋）。所得重组质粒ｐｃＤ－ＮＡ－ｇＤ以电穿孔法转染ＣＨＯ细胞，并以荧光染色法鉴定表达效果。用ｐｃＤＮＡ－ｇＤ免疫小鼠，ＥＬＩＳＡ法检测基因免疫
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| 关 键 词： | HSV－1 糖蛋白D 真核表达 基因免疫 载体构建 |
| 修稿时间： | 2000-03-21 |
Construction and its immunization effect of the eukaryotic expression vector bearing HSV-1 glycoprotein D gene |
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| YU Ying,MA Wen yu,YANG Qiao xin.Construction and its immunization effect of the eukaryotic expression vector bearing HSV-1 glycoprotein D gene[J].Journal of Cellular and Molecular Immunology,2000,16(4):308-310. |
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| Authors: | YU Ying MA Wen yu YANG Qiao xin |
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| Institution: | YU Ying,MA Wen yu,YANG Qiao xinDepartment of Microbiology,Faculty of Preclinical Medicine,Fourth Military Medical University,Xi'an 710032,Shaanxi Province,China |
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| Abstract: | Aim To construct eukaryotic expression vector bearing HSV-1 glycoprotein D(gD) gene and to evaluate the possibility of designing HSV-1 gene vaccine with HSV gD gene. Methods The HSV 1 gD gene was amplified from the HSV 1 genome DNA and cloned into eukaryotic vector pcDNA3.1( ). The recombinant vector pcDNA gD was transfected into CHO cells by electroperforance method. Fluorescent staining and ELISA were used to test the expression of gD in CHO cells and response of mice to pcDNA gD. Results The eukaryotic expression vector containing HSV 1 gD gene was successfully constructed .The recombinant vector could induce higher specific immunoreaction in the immunized mice. Conclusion The recombinant vector pcDNA gD is valuable to designing HSV gene vaccine and studying epitope biology and epitope vaccine of HSV 1. |
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| Keywords: | herpes simplex virus 1 glycoprotein D eu karyotic expression gene immunization |
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