骨肉瘤端粒酶的表达及其与临床的关系

目的 研究骨肉瘤端粒酶的表达,探讨骨肉瘤端粒酶的表达与临床特征的相关性。方法 应用原位杂交方法,检测49例骨肉瘤石蜡标本及10例正常骨组织和10例骨软骨瘤石蜡标本的端粒酶表达活性,并分析骨肉瘤的端粒酶表达活性与临床病理特征的关系。结果 49例骨肉瘤中端粒酶表达阳性39例(阳性率79.6％),而正常骨组织和骨软骨瘤端粒酶阳性表达率分别为0％和10％,与骨肉瘤比较差异极显著(P=0.001);骨肉瘤端粒酶的表达与肿瘤的大小、病理分级、3年存活率等明显相关。结论 端粒酶在骨肉瘤中阳性表达高,有辅助早期诊断的意义;骨肉瘤端粒酶的表达情况可以作为骨肉瘤预后评判的有效指标。

The expression of telomerase and its relation with clinical character of osteosarcoma

Objective To study the expression of the telomerase, and to evaluate the relations with clinical character of osteosarcoma. Methods With in situ hybridization, the expression of telomerase were observed in 49 paraffin sections of osteosarcoma, 10 paraffin sections of normal bone tissues and 10 paraffin sections of osteochondroma. Furthermore, the relationship of telomerase activity and patient's clinical character were analyzed. Results The telomerase was detected in 79.6% (39/49) of osteosarcoma cases, while in normal bone tissues and osteochondroma, the positive rates of telomerase were only 0% and 10% respectively. The positive detection of telomerase expression in osteosarcoma were significantly different from those in normal bone tissues and osteochondroma (P=0.001) The expression level of telomerase activity in osteosarcoma correlated well with tumor's location, surgical stage, pathological grade and the patient's prognosis. Conclusions Telomerase was significantly detected in osteosarcoma and may be a potential biochemical marker of diagnosis and prognosis for osteosarcoma; it seems to play an important role in the genesis and development of osteosarcoma.