An improved method for quantitative culture of Malassezia furfur.

Quantitative culture of Malassezia furfur from clinically healthy skin in 25 individuals was performed with two different methods using contact plates. The best results were obtained when a glucose peptone yeast extract medium, with the addition of milk, Tween-60, glycerol and glycerol monostearate was used. Different techniques for incubation and the reproducibility of this method were evaluated. Incubation can be done in a plastic bag at 32 or 37 degrees C. This new method is simple, the colonies are easy to identify and the counts are high and reliable.