脐血来源树突状细胞的培养鉴定

目的：探索体外诱导出的成熟脐血来源树突状细胞(CBDCs)功能性抗原表达的变化规律。方法：用光镜观察培养12dCBDCs的形态。用流式细胞仪分析培养不同时间CBDCs功能性抗原表达的变化规律。结果：体外诱导出典型形态的CBDCs，HLA-ABC、HLA-DR、CD1a在培养0～8d表达率渐升高，在第12d达高峰，第16d表达有所下降；CD83、CD80在第12d的表达略高于其他时间段；CD54在0d、4d、8d的表达率相近，而第12d的表达率明显升高，第16d明显降低。结论：应用本的方法，在体外培养第12d，能诱导出典型形态的CBDCs，多种功能性抗原的表达达到高峰。

Culture and Observation of Cord Blood Dendrite Cells

Objective:To explore the expression of functional antigens of cord bliid dendrite cells(CBDCs) induced in vitro. Methods: The morphology of CBDCs was observed under optic microscopy and the CBDC functional antigen expression at different time points was analysed by flow cytometry. Results;The form of CBDCs became typical on day 12. Expression of HLA-ABC,HLA-DR and CD1a increased gradually during days 0 ~8 at culture,reached peak on day 12, and began to decine on day 16. Expression of CD83, CD80 and CD54 was also peaked on day 12. Conclusion; Using methods in this study, CBDCs with typical form and varied functional antigens with peaked expression were noted on day 12.