用流式细胞仪分析骨髓细胞诊断淋巴瘤

FCM已经成为临床诊断淋巴瘤的有力工具.骨髓细胞FCM诊断的主要依据是SSC和CD_(45)细胞分布图上是否出现大小和分布不同于正常细胞的异常细胞,这种细胞是否存在和缺失相应的细胞表型.B细胞淋巴瘤的克隆性用免疫球蛋白к或λ轻链限制性确定,然后根据CD_5和CD_(10)表达与否,分为4种类型,进一步区分B细胞淋巴瘤亚型.T细胞淋巴瘤的克隆性用TCRα/β和γ/δ,TCR Vβ 25个家族的限制性确定,然后根据CD_4和CD_8表达与否,分为4种类型,进一步区分T细胞淋巴瘤亚型.NK细胞瘤可用CD_(56)、EBV、细胞毒颗粒TIA-1、颗粒酶B和穿孔素阳性与否进一步确定亚型.

Analysis of bone marrow cells by flow cytometry for diagnosis of lymphoma

FCM has become a powerful tool for clinical diagnosis of lymphoma.Bone marrow diagnosis by FCM was based primarily on whether there were the abnormal clones.whose size and distribution were different from normal cells in CD_(45)-versus-SSC plot.The corresponding phenotypes were present or lost in these clones.The monoclonality of diagnostic of B-cell lymphoma depends on the restriction of immunoglobulin kappa or lambda light chain.Then,based on the expression of CD_5 and CD_(10) or not,the B-cell lymphoma is divided into 4 types to further determine B lymphoma subtype.The monoclonality of diagnostic of T-cell lymphoma depends on the restriction of TCR α/β or γ/δ and 25 families.