自体骨髓间充质干细胞对异基因猪移植肝的保护作用

摘要 目的 研究自体骨髓间充质干细胞术中经门静脉输注对异基因小型猪移植肝脏的保护作用.方法 建立异基因猪肝移植急性排斥反应模型, 实验分对照组和处理组,对照组只做原位肝移植,处理组在原位肝移植的基础上,术中经门静脉注射一定数量预先制备的受体自身骨髓间充质干细胞,术后1d,3d,6d静脉采血检测肝功能,并做肝组织的病理活检 ,并观察受体的生存时间. 结果 1、处理组中受体的生存时间明显比对照组的延长P<0.001。2、肝功能变化：术前两组血清AST、ALT比较尚无统计学差异（p>0.05），术后处理组受体血清中AST,AL T总体变化明显低于对照组,两组比较有显著的统计学差异P<0.01；3、组织病理变化：处理组在术后7天病理活检见：大致呈正常肝脏病理, 肝组织轻度淤血，血管周围少量炎性细胞浸润, 少数汇管区小静脉或肝静脉内皮细胞下淋巴细胞浸润，肝细胞点状坏死，中性粒细胞浸润. 而对照组在术后3天移植的肝脏中出现汇管区大量炎症细胞聚集，肝细胞浑浊，气球样变性并出现不同程度的点状、灶状坏死。结论 自体骨髓间充质干细胞经门静脉输注能够保护移植的肝脏，延长受体的存活时间.

The protection of autologous bone marrow mesenchymal stem cells on transplanted liver of allogeneic mini-pigs

Abstract] Objective To study the protection of autologous bone marrow mesenchymal stem cells infused from portal vein for transplanted liver of allogeneic mini-pigs. Method established allogeneic liver transplantation acute rejection model, there were two groups: control group and treatment group, control group only made orthotopic liver transplantation, based on orthotopic liver transplantation in treatment group, injected a certain number of pre-receptor own bone marrow mesenchymal stem cells from portal vein during the operation, we detected liver function after 1day, 3days, 6days, and so the liver tissue biopsy, and the survival time of the receptor. Result 1, receptor in treatment group has a longer living time than that in the control group (P<0.001). 2, liver function changes: Preoperative Serum AST, ALT comparison had no statistical difference (p> 0.05), after operation treatment group receptor’s serum AST, ALT overall changes were obviously lower than the control group’s, the two groups showed a significant statistical difference P <0.01;3, histopathological changes: in the treatment group pathological changes 7days after operation show :roughly normal liver tissue , hepatic tissue has a little congestion, a little inflammatory cell infiltrate around blood vessel ,liver cell shows punctiform cellular necrosis, neutrophile granulocyte infiltrate. In control group a lot of lymphocyte aggregate in the header domain in the Transplanted liver, hepatic cell turbid and ballooning degeneration, we find large lobe cellular necrosis. Conclusion autologous bone marrow mesenchymal stem cells infused from portal vein could protect the transplanted liver, and extended the survival time of receptor.