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| 血清维生素E的测定(分光光度法)及其意义 | |
| 引用本文： | 刘时中,赖慕贤,林壮基. 血清维生素E的测定(分光光度法)及其意义[J]. 营养学报, 1987, 0(4) |
| 作者姓名： | 刘时中 赖慕贤 林壮基 |
| 作者单位： | 广东医药学院生化教研室 广州(刘时中，赖慕贤)，广东医药学院生化教研室 广州(林壮基) |
| 摘 要： | 维生素E(VE,即生育酚)是天然脂溶性抗氧化剂,对机体有一定的保护作用。摄入不足或某些疾病时,血清VE含量降低,服用VE则血清VE含量升高。 VE使Fe~(3+),还原成Fe~(2+),Fe~(2+)与联苯菲哕啉结合成粉红色复合物的分光光度法,方法简便,回收率及重复性较好,适于一般实验室应用。文中讨论了一些影响测定的因素。 |
| 关 键 词： | 血清维生素E 联苯菲啰啉 分光光度法 |
SPECTROPHOTOMETRIC DETERMINATION OF VITAMIN E AND ITS SIGNIFICANCE |
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| Liu Shizhong,Lai Muxian,Lin Zhuangji. SPECTROPHOTOMETRIC DETERMINATION OF VITAMIN E AND ITS SIGNIFICANCE[J]. Acta Nutrimenta Sinica, 1987, 0(4) | |
| Authors: | Liu Shizhong Lai Muxian Lin Zhuangji |
| Abstract: | A modified rapid microprocedure for the determination of VE in serum, was described. VE reduced Fe3 to Fe2 and then the Fe2 combined with bathophenanthroline to form a pink complex -which could be determined spect-rophotometrically. As little as 0.3ml (or 0.2ml) of serum or plasma sample was sufficient for determination. The reproducibility of this method was good. The CV was 4.1% (n=15). The recoveries of added VE (0.8, 0.2 and 1.6 mg/dl) were 86.3-102.5%. The mean value and SD of 99 normal adults were 1.15 0.31mg/dl. This spectrophotometric method was simple and could be used in common, laboratories. Some affecting frctors were discussed. |
| Keywords: | spectrophotometric determination vitamin E |
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