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| 首次应用酶联免疫法检测血浆纤维结合蛋白 | |
| 引用本文： | 张超. 首次应用酶联免疫法检测血浆纤维结合蛋白[J]. 中国基层医药, 1999, 0(4) |
| 作者姓名： | 张超 |
| 作者单位： | 淮南工业学院医学分院 232001 |
| 摘 要： | 目的 探讨血清纤维结合蛋白(Fn)含量的检测方法。方法 首次应用酶联免疫法(ELISA)检测待测血清,测定其光密度(D值),判定待测血清中Fn的含量。结果 该方法线性范围50～300mg/L,批内、日内、日间的CV分别为2.37％、2.89％、3.24％;回收率98.79％。结论 该方法线性范围适宜,准确度、精密度较好,符合临床检验工作要求。 |
| 关 键 词： | ELISA 纤维结合蛋白 |
The plasma fibronectin were first detected by ELISA |
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| ZHANG Chao. Medical College,Huainan Industrial Institute,Huainan,Anhui. The plasma fibronectin were first detected by ELISA[J]. Chinese Journal of Primary Medicine and Pharmacy, 1999, 0(4) | |
| Authors: | ZHANG Chao. Medical College Huainan Industrial Institute Huainan Anhui |
| Affiliation: | ZHANG Chao. Medical College,Huainan Industrial Institute,Huainan,Anhui 232001 |
| Abstract: | Objective To study the method of detecting fibronectin(Fn) content in serum. Methods The Fn content and its value of OD in serum were first detected by ELISA. Results The linear range of the method was 50 - 300 mg/L.The coefficient of variation(CV) within a batch, within a day and between days were 2.37% ,2. 89% and 3. 24% respectively. The rate of recovery was 98. 79% . Conclusion The linear range of this method is temperate. The accuracy and precision of this method are all higher and coincide with the request of clincal testing. |
| Keywords: | ELISA Fibronectin(Fn) |
| 本文献已被 CNKI 等数据库收录！ | |