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超抗原SEB及MBP诱导活化的淋巴细胞凋亡
摘    要:
目的 :探讨超抗原金黄色葡萄球菌肠毒素 B(SEB)及髓磷脂碱性蛋白 (MBP)是诱导静息成熟的淋巴细胞凋亡 ,还是诱导活化的淋巴细胞凋亡。方法 :采用 3H- Td R掺入法、Brd U掺入及其免疫组化法动态观察了小鼠脾脏和肠系膜淋巴结中淋巴细胞的增殖反应及其 Brd U阳性细胞的形态学变化。结果 :SEB、MBP注射 2 d时淋巴细胞增殖明显 ,Brd U阳性的细胞多数是核大的淋巴母细胞 ;注射 4d时淋巴细胞增殖明显减弱 ,Brd U阳性的细胞多数是核浓缩或核断裂的凋亡细胞。而SEB、MBP和对照组中的 Brd U阴性细胞核大小基本均匀一致 ,并且对照组淋巴细胞无明显的增殖反应。结论 :SEB、MBP是诱导活化的淋巴细胞凋亡

关 键 词:超抗原  BrdU  淋巴细胞凋亡

Apoptosis in activated lymphocytes induced by superantigen SEB and MBP
Xiao Xueyuan,Yang Guizhen. Apoptosis in activated lymphocytes induced by superantigen SEB and MBP[J]. Journal of Jilin University: Med Ed, 1998, 0(5)
Authors:Xiao Xueyuan  Yang Guizhen
Affiliation:Department of Immunology
Abstract:
Objective:To investigate apoptosis inducled by superantigen SEB and ABP in activated or resting lymphocytes.Methods:The method of H 3 TdR mix,BrdU mix and its immunohistochemistry were used to observe kineticly the proliferation of lymphocytes in spleen and mesenteric lymphnode of mice, as weel as the morphological changes in BrdU positive lymphocytes.Results:The results show that not only the lymphocytes proliferated markly,but also lymphoid blastocytes appeared in most of BrdU + cells after SEB and MBP 2 days injection.By contrast,the lymphocytes treated by SEB and MBP for 4 days proliferated weakly,and most of BrdU + cells were apoptotic lymphocytes.The lymphocytes had neigher proliferation nor BrdU + apoptotic cells in control group.Conclusion:SEB and MBP can induce apoptosis in activated lymphocytes only.
Keywords:superantigen  BrdU  lymphocytes apoptosis
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