HIV/AIDS患者血清IL-18浓度变化与辅助受体CCR5/CXCR4表达的关系研究

目的 研究人类免疫缺陷病毒／获得性免疫缺陷综合征（HIV／AIDS）患者血清白细胞介质（IL）-18浓度的变化，及其与病毒载量、CD4^＋T细胞表面辅助受体CCR5／CXCR4表达的相关性。方法 应用酶联免疫吸附试验（ELISA）对42例HIV／AIDS患者及12例正常对照者的血清IL-18浓度进行定量检测，分析其变化，并对其变化与病毒载量的相关性进行分析。应用流式细胞仪检测全部HIV／AIDS患者CD4^＋ T细胞表面辅助受体CCR5／CXCR4的表达情况，并分析其与病毒载量及血清IL-18浓度变化之间的相关性。结果 42例HIV／AIDS患者血清平均IL-18浓度显著高于正常对照组（P〈0．01），且按美国疾病控制与预防中心（CDC）1993年标准，CDCC期显著高于A期（P〈0．01）。所有患者血清IL-18浓度变化与HIV病毒载量之间呈正相关（P〈0．05），与辅助受体CX-CR4的表达也呈正相关（P〈0．01）。HIV病毒载量与辅助受体CXCR4的表达具有正相关性（P〈0．05）。结论 HIV／AIDS患者体内持续增长的IL-18加剧了其免疫功能的紊乱和低下，IL-18可能与疾病本身的致病机制有关。

Correlation between the expression of CCR5/CXCR4 and raised serum levels of interleukin-18 in HIV carriers/AIDS patients

Objective To investigate the serum IL-18 levels in HIV carriers/AIDS patients and their relations with viral load and the expression of coreceptors CCR5/CXCR4 on CD+4T lymphocytes.Methods Serum IL-18 levels of 42 HIV carriers/AIDS patients and 12 healthy controls were determined by ELISA,and their relations with serum viral load were analyzed.Flow-cytometry was used to detect the coreceptors CCR5/CXCR4 expressions on CD+4T lymphocytes in whole blood samples from 42 HIV carriers/AIDS patients.The correlation of the coreceptors expression with serum IL-18 levels and viral load was analyzed.Results Forty two HIV carriers/AIDS patients had significantly raised IL-18 levels compared with healthy controls(P<0.01),and there was significantly higher serum IL-18 levels in AIDS group C than AIDS in AIDS group A according to 1993 CDC standard(USA)(P<0.01).A significantly positive correlation among serum IL-18 levels,viral load and the CXCR4 expression on CD+4T lymphocytes was identified(P<0.05).Conclusions The persistently raised serum IL-18 levels in HIV carriers/AIDS patients will possibly exacerbate their immunodeficiency status,and may also be involved in the pathogenesis of the disorder itself.