| 卵巢癌细胞系Smac基因的表达及意义 |
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| 引用本文： | 丛江琳,王泽华,刘日明.卵巢癌细胞系Smac基因的表达及意义[J].华中医学杂志,2006,30(6):451-452. |
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| 作者姓名： | 丛江琳 王泽华 刘日明 |
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| 作者单位： | 1. 430022,武汉,华中科技大学同济医学院附属协和医院妇产科
2. 264000,烟台,青岛大学医学院附属烟台毓璜顶医院检验科 |
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| 摘 要： | 目的 探讨Smac基因在卵巢癌细胞系A2780、COC1及其顺铂(DDP)耐药株A2780/DDP、COC1/DDP中的表达及其意义.方法 半定量逆转录-聚合酶链反应和免疫印迹法检测卵巢癌细胞A2780、COC1及其顺铂耐药株A2780/DDP、COC1/DDP中Smac mRNA和蛋白的表达.结果 卵巢癌细胞A2780、COC1及其顺铂耐药株A2780/DDP、COC1/DDP四种细胞系中均有Smac mRNA和蛋白的表达;Smac在A2780、COC1中的表达高于其顺铂耐药株.结论 低表达Smac抑制凋亡,低表达Smac可能与耐药有关.
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| 关 键 词： | 卵巢癌 细胞 基因 逆转录-聚合酶链反应 |
| 修稿时间： | 2006年6月30日 |
Expression and Significance of Smac gene in ovarian carcinoma cell lines |
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| Cong Jianglin,Wang Zehua,Liu Riming.Expression and Significance of Smac gene in ovarian carcinoma cell lines[J].Central China Medical Journal,2006,30(6):451-452. |
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| Authors: | Cong Jianglin Wang Zehua Liu Riming |
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| Abstract: | Objective To examine the expression and significance of Smac gene in the ovarian carcinoma cell lines A2780,COC1 and their cisplatin resistant cell lines A2780/DDP,COC1/DDP.Methods The semi-quantitative RT-PCR method was used to detect the relatively quantity of Smac mRNA in the ovarian carcinoma cell lines A2780,COC1 and their cisplatin resistant cell lines;western blot was used to detect the expression of Smac protein.Results The expressions of Smac mRNA and protein in A2780 and COC1 were much more than in A2780/DDP cell line and COC1/DDP cell line;the difference is significiant.Conclusion Downregulated expression of Smac gene may be inhibit apoptosis.It also may be related to the mechanism of drug resistance. |
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| Keywords: | Ovarian carcinome Cell Smac gene Western blot |
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