金刚丸对骨质疏松大鼠骨代谢和血清骨钙素的影响

目的 探讨金刚丸对骨质疏松大鼠骨代谢和血清骨钙素的影响.方法 将60只SPF级大鼠随机分为正常对照组、模型组及高、中、低剂量组和己烯雌酚组,每组10只.高、中、低剂量金刚丸组每天分别以3.24、1.62、0.81 g/kg的剂量灌胃1次;己烯雌酚组大鼠每天给予1.0 mg/kg灌胃1次;正常对照组及模型组给予等体积的生...

Effects of Jingang Pill on bone metabolism and serum osteocalcin in osteoporosis rats

Objective To investigate the effects of Jingang Pill on bone metabolism and serum osteocalcin in osteoporosis rats. Methods 60 SPF rats were randomly divided into control group, model group, high, medium and low dose Jingang Pill groups and diethylstilbestrol group, with 10 rats in each group. The high, medium and low dose Jingang Pill groups were given intragastric administration of 3.24, 1.62, 0.81 g/kg per day respectively;the rats in the diethylstilbestrol group were given diethylstilbestrol1.0 mg/kg every day;rats in the control group and model group were given equal volume of normal saline. After 6 weeks of administration,the femur was taken to detect the maximum load and fracture load;bone trabecular structure was observed by HE staining;the levels of serum bone alkaline phosphatase(BALP), estradiol(E2) and serum bone gla-containing protein(BGP), osteoprotegerin(OPG),receptor activator of NF-κB(RANK) and receptor activator of NF-κB ligand(RANKL) were measured by ELISA;bone mineral density(BMD) was measured by BMD tester;the relative expression of transient receptor potential cation channel subfamily V member 6(TRPV6) protein was detected by Western blot. Results In the model group, bone trabecular morphology was blurred and extensively fractured, bone marrow cavity was widened, and bone cells were scattered. Compared with the model group, bone trabecular morphology and structure were improved in high, medium and low dose groups, bone marrow cavity was reduced, and bone membrane arrangement was improved. Compared with the control group, the levels of body weight, BALP, BGP, RANK and RANKL in model group were significantly increased, while the levels of E2, BMD, maximum load, breaking load, OPG and TRPV6 were significantly decreased(P <0.05). Compared with the model group, the levels of body weight, BALP, BGP, RANK and RANKL in medium and high dose groups were significantly increased, while the levels of E2, BMD, maximum load, breaking load, OPG and TRPV6 were significantly decreased(P<0.05). Conclusion Jingang Pill has the effect of anti-osteoporosis and bone protection by increasing the estrogen level, regulating bone metabolism, improving biomechanical properties and BMD in postmenopausal osteoporosis rats, and mechanism may be through affecting the expression of TRPV6, so as to up-regulate the expression of bone protective protein and down-regulate the expression of RANKL.