桂枝芍药知母汤提取工艺的优化

目的 优化桂枝芍药知母汤(GSZD)的提取工艺.方法 采用高效液相色谱法同时测定GSZD中盐酸麻黄碱、盐酸伪麻黄碱、芒果苷、芍药苷、甘草苷、5-O-甲基维斯阿米醇苷、甘草酸、肉桂酸、6-姜酚9个成分的含量.在单因素实验的基础上,以料液比、提取次数、提取时间为考察因素,以上述9个成分含量和干膏得率作为评价指标,采用层次分...

Optimization of extraction technology of Guizhi shaoyao zhimu decoction

OBJECTIVE To optimize the extraction technology of Guizhi shaoyao zhimu decoction(GSZD).METHODS The contents of 9 components in GSZD were determined by HPLC,such as ephedrine hydrochloride,pseudoephedrine hydrochloride,mangiferin,paeoniflorin,liquiritin,5-O-methylvisammioside,glycyrrhizic acid,cinnamic acid,6-gingerol.On the basis of single factor experiment,taking material-liquid ratio,extraction times and extraction time as inspection factors,taking the contents of above 9 components and the yield of dry extract as evaluation indicators,the analytic hierarchy process and entropy weight method were used to determine the composite weight of each index and calculate the comprehensive score;the extraction technology parameters of GSZD were optimized by Box-Behnken response surface method,and the validation tests were conducted.RESULTS The composite weight of the contents of ephedrine hydrochloride,pseudoephedrine hydrochloride,mangiferin,paeoniflorin,glycyrrhizin,5-O-methylvisa-midol,glycyrrhizinate,cinnamic acid,6-gingerol and the yield of dry extract were respectively 0.12,0.10,0.05,0.12,0.14,0.06,0.13,0.15,0.10,0.03.The optimal extraction technology of GSZD is that the ratio of material to liquid is 1∶14(g/mL),extraction is 2 times,and the extraction time is 3.0 h;average comprehensive score of the 3 verification tests was 95.879,and RSD was 0.50%(n=3),the deviation from the predicted comprehensive score(94.328)was 1.64%.CONCLUSIONS In this study,the optimal extraction technology of GSZD is determined.