Mutations in four chloroplast loci of Chlamydomonas reinhadtii affecting the photosystem I reaction centers

Summary In this work seven chloroplast mutations conferring a deficiency in photosystem I reaction centers have been mapped at four chloroplast loci in Chlamydomonas reinhardtii. Recombination frequencies were estimated from diploid progeny of vegetative zygotes. These four loci were scattered throughout the chloroplast genome. The three mutations at locus I were found to be tightly linked to a mutation in the rbcL gene coding for the large subunit of ribulose-1,5-bisphosphate carboxylase (Dron et al. 1983). As the psaA2 gene coding for one apoprotein of the chlorophyll-complex CPI, identified by its homology with the corresponding maize gene (Fish et al. 1985), has been found close to the rbcL gene (Dron et al. 1982), the psaA2 gene could be at locus 1.