Quantitative gene expression analysis in microdissected archival tissue by real-time RT-PCR |
| |
| Authors: | Specht K Richter T Müller U Walch A Höfler M W |
| |
| Affiliation: | GSF-Forschungszentrum für Umwelt und Gesundheit, Institut für Pathologie, Neuherberg, Germany. |
| |
| Abstract: | ![]()
Laser-assisted microdissection is a powerful tool for the analysis of morphologically defined cell populations. We report here that the combination of microdissection and real-time RT-PCR technologies together with an optimized RNA microscale extraction procedure allows the reproducible determination of gene expression levels in archival formalin-fixed, paraffin-embedded tissue samples. |
| |
| Keywords: | |
| 本文献已被 PubMed 等数据库收录！ |
|
