| 用BA－ELISA斑点法检测痢疾杆菌免疫小鼠GALT中的特异性抗体分泌细胞（ASC） |
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| 引用本文： | 赖燕来,高杰英.用BA－ELISA斑点法检测痢疾杆菌免疫小鼠GALT中的特异性抗体分泌细胞（ASC）[J].中国免疫学杂志,1995(5). |
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| 作者姓名： | 赖燕来 高杰英 |
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| 作者单位： | 军事医学科学院微生物流行病研究所 |
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| 摘 要： | 改进的ＢＡ－ＥＬＩＳＰＯＴ法使免疫酶斑更为清晰，保存时间延长。用此法检测了痢疾杆菌福氏２ａ经口及腹腔免疫后，小鼠派伊尔氏（ＰＰ）淋巴结、肠系膜淋巴结（ＭＬＮ）及脾脏（ＳＰＬ）中特异性ＩｇＡ、ＩｇＧ、ＩｇＭ抗体分泌细胞（ＡｎｔｉｂｏｄｙＳｅｃｒｅｔｉｎｇｃｅｌｌ，ＡＳＣ）的动态变化，得到有规律的结果：两种免疫途径均能在ＰＰ及ＭＬＮ中诱导出３类特异ＡＳＣ的显著升高，但口服导致的升高其持续时间较腹腔途径为短。此外，腹腔途径还能诱导ＳＰＬ中３种ＡＳＣ升高。
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| 关 键 词： | 痢疾杆菌福氏2a，BA－ELISPOT，抗体分泌细胞（ASC），免疫应答 |
AN IMPROVED BA-ELISPOT ASSAY FOR THE DETECTION ANDENUMERATION OF
SPECIFIC ANTIBODY-SECRETING CELLS(ASC)IN GALT FROM THE SHIGELLA
FLEXNERI 2a INFECTEDMICE |
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| Ani Yanlai, Gao Jieying Institute of
Microbiology and Epidemiology,academy of MilitoryMedical
Sciences,Beijing.AN IMPROVED BA-ELISPOT ASSAY FOR THE DETECTION ANDENUMERATION OF
SPECIFIC ANTIBODY-SECRETING CELLS(ASC)IN GALT FROM THE SHIGELLA
FLEXNERI 2a INFECTEDMICE[J].Chinese Journal of Immunology,1995(5). |
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| Authors: | Ani Yanlai Gao Jieying Institute of
Microbiology and Epidemiology academy of MilitoryMedical
Sciences Beijing |
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| Institution: | Ani Yanlai, Gao Jieying Institute of
Microbiology and Epidemiology,academy of MilitoryMedical
Sciences,Beijing 100850 |
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| Abstract: | |
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| Keywords: | S flexneri 2a BA-ELISPOT assay ASC Immune response |
| 本文献已被 CNKI 等数据库收录！ |
