Comparison of different sampling techniques and of different culture methods for detection of group B streptococcus carriage in pregnant women |
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| Authors: | Nabil A El Aila Inge Tency Geert Claeys Bart Saerens Piet Cools Hans Verstraelen Marleen Temmerman Rita Verhelst Mario Vaneechoutte |
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| Affiliation: | (1) Laboratory Bacteriology Research, Department of Clinical Chemistry, Microbiology & Immunology, University of Ghent, Ghent, Belgium;(2) Department of Obstetrics & Gynaecology, Ghent University Hospital, University of Ghent, Ghent, Belgium;(3) Laboratory for Clinical Biology, Ghent University Hospital, University of Ghent, Ghent, Belgium |
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| Abstract: | Background Streptococcus agalactiae (group B streptococcus; GBS) is a significant cause of perinatal and neonatal infections worldwide. To detect GBS colonization in pregnant women, the CDC recommends isolation of the bacterium from vaginal and anorectal swab samples by growth in a selective enrichment medium, such as Lim broth (Todd-Hewitt broth supplemented with selective antibiotics), followed by subculture on sheep blood agar. However, this procedure may require 48 h to complete. We compared different sampling and culture techniques for the detection of GBS. |
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