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| 应用聚合酶链反应快速检测支气管肺泡灌洗液中的结核菌 | |
| 引用本文： | 董德琼 杨渝浩. 应用聚合酶链反应快速检测支气管肺泡灌洗液中的结核菌[J]. 遵义医学院学报, 1997, 20(4): 23-25 |
| 作者姓名： | 董德琼 杨渝浩 |
| 作者单位： | 遵义医学院第一附院内科(董德琼，杨渝浩，申茂权，凌建华)，遵义医学院第一附院检验科(彭理年，肖瑜) |
| 摘 要： | 目的：本研究评价聚合酶链反应（PCR）技术检测支气管肺泡准洗液中结核杆菌DNA的作用。方法：应用PCR技术对161例（肺结核81例，非肺结核80例作为对照）支气管肺泡灌洗液（BALF）进行结核菌DNA检测。结果：结核组BALF的阳性率：PCR为69．l％、徐片抗酸染色为86％、培养为98％，PCR的阳性率与涂片和培养比较，差异有显著性（P＜0．01）。非结核对照组BALF的阳性率为：PCR2.5％，涂片0％，培养0％。结论：PCR技术测定BALF中的结核菌是诊断肺结核的一种快速、敏感、特异的方法，尤其适用于无痰的患者。 |
| 关 键 词： | 聚合酶链反应 结核杆菌 支气管肺泡灌洗 |
Rapid detection of M. tuberculosis in Bronchoalveolar lavage fluids specimens by polymerase chain reaction |
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| Dong Deqing , Yang Yuhao, Peng Linian ,et al.. Rapid detection of M. tuberculosis in Bronchoalveolar lavage fluids specimens by polymerase chain reaction[J]. Acta Academiae Medicine Zunyi, 1997, 20(4): 23-25 | |
| Authors: | Dong Deqing Yang Yuhao Peng Linian et al. |
| Affiliation: | Department of Medicine ; Affiliated Hospital of Zunyi Medial College. |
| Abstract: | Objective: To evaluate the value of Polymerase chain reacion (PCR) technique indetecting DNA of mycobactierium tuberculosis in bronchoalveolar lavage fluids (BALF).Methods : PCR was applied to detect DNA of in 161 patients (81 with pulmonare tuberculosisand 80 as control). Results: In 81 patients with pulmonary tubdectulosis, the positive rates ofDNA in BALF by PCR was 69. 1 % ; smear fast-acid staining 8. 6% and culture 9. 8% thePositive rates of PCR in BALF are significanily higher than that of smears and culture methods(P < 0. 01 ). In non-pulmonary tuberculosis series ; the positive rates of DNA in BAir by PCRwas 2 .5% smear 0% and culture 0%. Conclusions: detecting M. tuberculosis in BALF byPCR is a rapid. sensitive. specifc method for diagnsing Pulmonary tubereulosis and is especialyadepted to those patients without sputum. |
| Keywords: | polymerase chain reaction bronchoalveolar lavage mycobacterium tuberculosis |
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