Comparison of complete nucleotide sequences and genome organization of six distinct cherry leaf roll virus isolates from New Zealand

The complete genomic sequences of six phenotypically distinct cherry leaf roll virus (CLRV) isolates from New Zealand were determined and compared. RNA1 and RNA2 are 7,919-7,921 and 6,361-6,363 nucleotides in length, respectively, excluding the poly(A) tails. Both genome segments contain a single open reading frame and encode one polyprotein of 2,113 amino acids for RNA1 and 1,590 aa for RNA2, with corresponding molecular masses of 235.5-236.1 kDa and 174.2-174.8 kDa, respectively. The six RNA1 sequences share 92.0-99.7 % nt and 97.0-99.5 % aa similarity, and the RNA2 sequences share 91.4-99.8 % nt and 94.3-99.7 % aa similarity. Phylogenetic analysis established close associations between the six New Zealand CLRV isolates and members of the subgroup C nepoviruses.