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A PCR-based microwell-plate hybrid capture assay for high-risk human papillomavirus |
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| Authors: | Yumei Wang Yan Liu Yaping Ding Nan Sun Yafang Gong Shangxian Gao |
| Affiliation: | 1. Department of In Vitro Diagnostic Reagents, Key Laboratory of the Ministry of Health for Research on Quality and Standardization of Biotech Products, National Institutes for Food and Drug Control, No. 2 Tiantanxili, Beijing, 100050, China 2. Shenzhen Kangmei Biotechnology Co., Ltd, Shenzhen, 518057, China 3. Third Xiangya Hospital of Central South University, Changsha, 410013, China |
| Abstract: | Human papillomavirus (HPV) is associated with cervical cancer. In this study, we developed a high-throughput microwell-plate hybrid capture (MPHC) method for epidemiological studies of high-risk HPV (HRHPV). The results with 1238 cervical specimens from female outpatients showed a concordance rate of 94.3 % between the MPHC and Hybrid Capture II assay. The MPHC assay showed an average HRHPV rate of 29.3 % for high-risk populations in populous cities of China. The established MPHC assay could sensitively and specifically detect 13 types of HRHPV and is suitable for large-scale screening, especially in areas where real-time PCR or fluorescence equipment is unavailable. |
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