Immunoblot analysis of c-Met expression in human colorectal cancer: Overexpression is associated with advanced stage cancer |
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Authors: | Zhaoshi?Zeng,Martin?R?Weiser,Matt?D’Alessio,Andrew?Grace,Jinru?Shia,Philip?B.?Paty author-information" > author-information__contact u-icon-before" > mailto:patyp@mskcc.org" title=" patyp@mskcc.org" itemprop=" email" data-track=" click" data-track-action=" Email author" data-track-label=" " >Email author |
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Affiliation: | (1) Colorectal Service, Department of Surgery, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, 10021 New York, NY, USA;(2) Department of Pathology, Memorial Sloan-Kettering Cancer Center, New York, New York, USA |
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Abstract: | c-Met, the receptor of hepatocyte growth factor is known to be responsible for the motility and mitogenesis of epithelial cells including cancer cells. To investigate the significance of c-Met expression in human colorectal cancer (CRC), total cellular protein, extracted from 130 CRCs were examined by Western blot analysis. The signal was quantitated by ChemiImager™ 4000 Low Light Imaging System. c-Met expression was analyzed as the ratio of tumor to matched normal tissue (T/N) and expressed as fold-increase. The cellular localization of c-Met was assessed by immunohistochemistry. The T/N fold increase of c-Met varied from 0.2 to 10.7 with a mean of 3.41 ± 0.23 (mean ± SE). 69% primary CRC showed overexpression (T/N >2.0) of c-Met. Significantly higher c-Met levels were found in CRC with blood vessel invasion (P = 0.04), and in advanced stage (P = 0.04). No relationship was noted between c-Met expression and age, tumor size, location, differentiation. C-Met immunoreactivity was observed in the membrane and cytoplasm of cancer cells. Positive staining of endothelial cells of blood vessels within normal submucosa and tumor was also evident. C-Met protein is expressed at levels significantly higher than adjacent mucosa in most primary adenocarcinomas of the colon. Our results support an important role for c-Met in human CRC progression and metastasis. |
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Keywords: | blood vessel invasion c-Met proto-oncogene colorectal cancer metastasis |
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