伽玛刀低剂量照射对致疒间大鼠海马中氨基酸递质的影响

目的观察伽玛刀低剂量照射对致疒间大鼠海马中谷氨酸(Glu)、γ-氨基丁酸(GABA)的影响,探讨伽玛刀治疗癫疒间的作用机制。方法将50只锂-匹罗卡品致疒间大鼠随机分为照射组和非照射组,各25只,另取正常大鼠25只(腹腔注射生理盐水)作为对照组。照射组大鼠进行伽玛刀低剂量照射(周边剂量20Gy),对照组和非照射组大鼠不进行伽玛刀照射。用高效液相色谱-质谱-质谱(HPLC-MS-MS)分析法检测各组大鼠海马Glu、GABA的含量。结果伽玛刀照射2周后,照射组和非照射组大鼠海马中Glu含量均高于对照组,照射组GLu含量低于非照射组,差异具有统计学意义(P0.05);照射组和非照射组GABA含量均低于对照组,照射组GABA含量高于非照射组,差异具有统计学意义(P0.05)。结论伽玛刀低剂量照射通过调节海马中Glu与GABA的平衡,以达到抗癫疒间的作用。

Effect of low-dose Gamma Knife radiation on amino acid transmitters in the hippocampus of epileptic rats

Objective To explore the mechanism of Gamma Knife radiosurgery for epilepsy by studying the effect of low-dose Gamma Knife radiosurgery on glutamate （Glu） and γ-aminobutyric acid （GABA） in the hippocampus of epileptic rats. Methods Fifty Li-pilocarpine-induced epileptic rats were randomly divided equally into radiation group and non-radiation group. Additional twenty-five normal rats were treated with normal sodium as the control group. The radiation group rats were treated by Gamma Knife radiation at a marginal dose of 20 Gy and no radiation was performed in non-radiation and control groups. Glu and GABA contents in the hippocampus of epileptic rats were detected by high performance liquid chromatography-mass spectrometry-mass spectrometry （HPLC-MS-MS） in all groups. Results After two weeks of radiation,the contents of Glu in the hippocampus of the rats in non-radiation and radiation groups were significantly higher than control group,and that of radiation group was significantly lower than non-irradiation group （P0.05）. The contents of GABA in the hippocampus of the rats in non-irradiated and irradiated group were significantly lower than control group,and that of radiation group was significantly higher than non-irradiated group （P0.05）. Conclusions Low-dose Gamma Knife irradiation can counteract epilepsy through the regulation of the balance of Glu and GABA in the hippocampus.