神经化组织工程骨构建的初步观察

目的评估两种组织工程骨体内神经化重建方法的成骨效果,研究神经化与成骨的相互关系。方法26只新西兰大白兔,其中24只随机分成四组:组织工程骨组(A组),感觉神经束植入组(B组),运动神经束植入组(C组),血管束植入组(D组);另2只为空白对照组。每只动物均制备左侧股骨长1.5cm的段缺性骨与骨膜缺损,钢板固定后骨缺损处分别植入用四种方法制备的组织工程骨。植入的神经分别是隐神经和股神经肌支。术后4、8、12周摄股骨正位X线片,用放射影像学评分和X线阻射影分析比较骨缺损修复情况。结果在组织工程骨中植入感觉神经束后,比单纯组织工程骨和运动神经束植入的修复效果均有明显提高,而在组织工程骨中植入运动神经束与单纯组织工程骨修复骨缺损的效果相比较无明显差异,感觉神经束植入与血管束植入的成骨效果比较无明显差异,血管束植入组的成骨效果优于其它两组。结论利用感觉神经束植入的方法可以提高组织工程骨的成骨作用,而植入运动神经束却无此作用。

A primary study on reconstruction of neurotization of tissue engineered bone

Objective To compare the effects of two methods of reconstruction of neurotization in vivo on osteogenesis of tissue-engineered bone.Methods 24of 26New Zealand rabbits were divided into 4groups ran-domly and equally:tissue-engineering group(A),sensory nerve tract group(B),motor nerve tract group(C),and vessel tract group(D).A segmental defect of 1.5cm long was made at the left femur in each animal.After plate fixation,the defects were implante d respectively with the engineered b ones prepared in the above-mentioned 4ways.The implanted nerves were taken resp ectively from saphenous nerves and muscular branches of femoral nerves.At 4,8and 12weeks postoperatively,ante roposterior radiographs were taken on the left femur in the 4groups.The reparative effects of bone were evaluated according to roentgenographi c analysis.Results After implantation of sensory nerve tract into the tissue-engineered bone,the reparative effect was obviously better than that in both group A and group C.No obvious osteogeneti c difference was found between group B and group D,but the osteogenetic effect in group D excelled that in the other two groups.No distinct difference was observed between the osteogenetic effects in group C and group A.Conclusion Implantation of sensory nerve tract into the tissue-engineered bone can improve its osteogenesis,but im plantation of the motor nerve tract c annot achieve the similar effect according to this experiment. [