Rapid detection of herpes simplex virus DNA by in situ hybridization with photobiotin-labelled double-stranded DNA probes |
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| Authors: | W Chantratita E A Henchal C Yoosook |
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| Affiliation: | Department of Microbiology, Faculty of Science, Mahidol University, Bangkok, Thailand. |
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| Abstract: | ![]()
An assay for rapid detection of herpes simplex virus in infected cells is described. The assay utilizes in situ hybridization with photobiotin-labelled double-stranded DNA probes prepared from HSV-1 DNA cloned in plasmid vectors. The assay provided an alternative method for earlier detection of virus in cell cultures with the ease of preparation of photobiotin-labelled double-stranded DNA. |
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